16-9856-82

antibody from Invitrogen Antibodies

Targeting: CLEC7A CD369, CLECSF12, dectin-1, hDectin-1, SCARE2

Provider product page for 16-9856-82

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Antibody data

Antibody Data
Antigen structure
References [6]
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Product number: 16-9856-82 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD369 (Clec7a, Dectin-1) Monoclonal Antibody (15E2), Functional Grade, eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: This 15E2 monoclonal antibody reacts with human and non-human primate CD369, which is also known as the Dectin-1 or beta-glucan receptor or CLEC7A. CD369 is expressed predominantly on monocytes, macrophages, and dendritic cells. Moreover, this receptor can also be detected on neutrophils and lymphocytes, as well as induced by cytokines such as IL-4 or LPS. In humans, CD369 exists as two isoforms that arise due to alternative splicing. CD369 binds beta-1,3-glucan and plays an important role in activating signal transduction involved in the innate immune response to fungal and mycobacterial infections. Studies have also shown the involvement of CD369 in dendritic cell activation. The 15E2 monoclonal antibody has been reported to recognize both isoforms of CD369 and activate dendritic cells. It does not crossreact to mouse CD369. Applications Reported: The 15E2 antibody has been reported to activate dendritic cells. Applications Tested: This 15E2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 15E2
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: 4° C

CLEC7A protein structure - 16-9856-82 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 Ca IG induces the transcription and expression of IL-6, TNFalpha involving the dectin-1-Syk pathways ( A ) RNA was harvested at 0, 1, 3, 6, 24 hours after treated with 100 mug/ml Ca IG and subjected to gene expression analysis by qRT-PCR normalized to beta-actin expression. For both genes analyzed, the significant difference of the Ca IG treated cells was compared with the corresponding untreated cells. ( B ) The culture medium of THP-1 cells treated with 100 mug/ml Ca IG for 24 hours was analyzed by ELISA to determine the protein level of IL-6 and TNFalpha. ( C ) The expression of dectin-1 in THP-1 cells. The THP-1 cells (2 x 10 5 cells) were incubated with anti-human primate Dectin-1 monoclonal antibody for flow cytometric analysis. ( D ) Representative images of Western Blot analyses of p-Syk, Syk, p-IkappaBalpha, IkappaBalpha, p-p38 and p38 protein levels. Protein extracts were made from untreated cells and from Ca IG treated cells for 30, 60 and 90 minutes. Extracted protein samples (50 mug per lane) were subjected to electrophoresis and immunoblotting with antibodies specific for the 6 proteins and beta-actin as control for equal loading. ( E ) NF-kappaB p65 translocation was analyzed by staining with NF-kappaB-p65 (red); and nucleuses were colored with DAPI (blue). Scale bar = 20 mum. ( F ) THP-1 cells were transfected with dectin-1 specific siRNA (siRNA-dectin-1) or scrambled control siRNA (NC) for 48 hours, and were subsequently treated with Ca IG for 6 hour

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FIGURE 5 Crispr/Cas knockout (KO) and of immune regulatory receptors in human induced pluripotent stem cells (hiPSCs) and functional analysis of macrophages derived from relative KO clones. (A) Workstream scheme for generating and analyzing of single cell KO clones in hiPSC. (B) Eight out-of-frame homozygous Dectin-1 KO clones were identified by Sanger sequencing and interference of CRISPR edits (ICE) analysis. (C) Macrophage marker expression level between wild type (WT) and Dectin-KO clone No. 32-derived macrophages. (D) Dectin-1 expression level and relative isotype in macrophages derived from WT and Dectin-1 KO clone No. 32.