17643-1-AP

antibody from Proteintech Group

Targeting: HMGCS1 HMGCS

Provider product page for 17643-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [32]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 17643-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#17643-1-AP, RRID:AB_2248359
Product name: HMGCS1 antibody
Antibody type: Polyclonal
Description: KD/KO validated HMGCS1 antibody (Cat. #17643-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

HMGCS1 protein structure - 17643-1-AP shown in red.

Submitted references Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism.

Zhao L, Qiu Z, Yang Z, Xu L, Pearce TM, Wu Q, Yang K, Li F, Saulnier O, Fei F, Yu H, Gimple RC, Varadharajan V, Liu J, Hendrikse LD, Fong V, Wang W, Zhang J, Lv D, Lee D, Lehrich BM, Jin C, Ouyang L, Dixit D, Wu H, Wang X, Sloan AE, Wang X, Huan T, Mark Brown J, Goldman SA, Taylor MD, Zhou S, Rich JN
Nature cancer 2024 Jan;5(1):147-166

Hypoxia upregulating ACSS2 enhances lipid metabolism reprogramming through HMGCS1 mediated PI3K/AKT/mTOR pathway to promote the progression of pancreatic neuroendocrine neoplasms.

Gu D, Ye M, Zhu G, Bai J, Chen J, Yan L, Yu P, Lu F, Hu C, Zhong Y, Liu P, He Q, Tang Q
Journal of translational medicine 2024 Jan 23;22(1):93

Complexes of Soluble Dietary Fiber and Polyphenols from Lotus Root Regulate High-Fat Diet-Induced Hyperlipidemia in Mice.

Zheng Z, Gao W, Zhu Z, Li S, Chen X, Cravotto G, Sui Y, Zhou L
Antioxidants (Basel, Switzerland) 2024 Apr 16;13(4)

CCR9 overexpression promotes T-ALL progression by enhancing cholesterol biosynthesis.

Jamal M, Lei Y, He H, Zeng X, Bangash HI, Xiao D, Shao L, Zhou F, Zhang Q
Frontiers in pharmacology 2023;14:1257289

miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway.

Xing K, Bian X, Shi D, Dong S, Zhou H, Xiao S, Bai J, Wu W
Journal of hepatocellular carcinoma 2023;10:2173-2185

Gypenosides suppress hepatocellular carcinoma cells by blocking cholesterol biosynthesis through inhibition of MVA pathway enzyme HMGCS1.

Xiao MY, Li FF, Xie P, Qi YS, Xie JB, Pei WJ, Luo HT, Guo M, Gu YL, Piao XL
Chemico-biological interactions 2023 Sep 25;383:110674

Morusinol Extracted from Morus alba Inhibits Cell Proliferation and Induces Autophagy via FOXO3a Nuclear Accumulation-Mediated Cholesterol Biosynthesis Obstruction in Colorectal Cancer.

Zhang X, Dong Z, Yang Y, Liu C, Li J, Sun W, Zhu Y, Shen Y, Wang Z, Lü M, Cui H
Journal of agricultural and food chemistry 2023 Nov 1;71(43):16016-16031

P4HA1 activates HMGCS1 to promote nasopharyngeal carcinoma ferroptosis resistance and progression.

Zhou R, Qiu L, Zhou L, Geng R, Yang S, Wu J
Cellular signalling 2023 May;105:110609

ERRα Up-Regulates Invadopodia Formation by Targeting HMGCS1 to Promote Endometrial Cancer Invasion and Metastasis.

Tang S, Ma J, Su P, Lei H, Tong Y, Cai L, Xu S, Mao X, Sun P
International journal of molecular sciences 2023 Feb 16;24(4)

Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis.

Zhou W, Liu H, Yuan Z, Zundell J, Towers M, Lin J, Lombardi S, Nie H, Murphy B, Yang T, Wang C, Liao L, Goldman AR, Kannan T, Kossenkov AV, Drapkin R, Montaner LJ, Claiborne DT, Zhang N, Wu S, Zhang R
Cancer cell 2023 Apr 10;41(4):740-756.e10

MiR-122-5p regulates the mevalonate pathway by targeting p53 in non-small cell lung cancer.

Zheng YK, Zhou ZS, Wang GZ, Tu JY, Cheng HB, Ma SZ, Ke C, Wang Y, Jian QP, Shu YH, Wu XW
Cell death & disease 2023 Apr 1;14(4):234

Phyllanthus Niruri L. Exerts Protective Effects Against the Calcium Oxalate-Induced Renal Injury via Ellgic Acid.

Li MT, Liu LL, Zhou Q, Huang LX, Shi YX, Hou JB, Lu HT, Yu B, Chen W, Guo ZY
Frontiers in pharmacology 2022;13:891788

Itraconazole Inhibits the Growth of Cutaneous Squamous Cell Carcinoma by Targeting HMGCS1/ACSL4 Axis.

Xu C, Zhuo Y, Liu Y, Chen H
Frontiers in pharmacology 2022;13:828983

Upregulation of Superenhancer-Driven LncRNA FASRL by USF1 Promotes De Novo Fatty Acid Biosynthesis to Exacerbate Hepatocellular Carcinoma.

Peng JY, Cai DK, Zeng RL, Zhang CY, Li GC, Chen SF, Yuan XQ, Peng L
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Oct 28;10(1):e2204711

Discovery and Application of Postnatal Nucleus Pulposus Progenitors Essential for Intervertebral Disc Homeostasis and Degeneration.

Gao B, Jiang B, Xing W, Xie Z, Luo Z, Zou W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 May;9(13):e2104888

Krüppel-like Factor 13 Promotes HCC Progression by Transcriptional Regulation of HMGCS1-mediated Cholesterol Synthesis.

Chen CC, Xie XM, Zhao XK, Zuo S, Li HY
Journal of clinical and translational hepatology 2022 Dec 28;10(6):1125-1137

miRNA-223 Suppresses Mouse Gallstone Formation by Targeting Key Transporters in Hepatobiliary Cholesterol Secretion Pathway.

Zhao F, Ma S, Zhou Y, Wei B, Hao Z, Cui X, Xing L, Liu G, Jin L, Ma T, Shi L
International journal of biological sciences 2021;17(15):4459-4473

Transcriptomic Analysis Reveals the Protective Effects of Empagliflozin on Lipid Metabolism in Nonalcoholic Fatty Liver Disease.

Ma Y, Kan C, Qiu H, Liu Y, Hou N, Han F, Shi J, Sun X
Frontiers in pharmacology 2021;12:793586

TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.

Ho WY, Chang JC, Lim K, Cazenave-Gassiot A, Nguyen AT, Foo JC, Muralidharan S, Viera-Ortiz A, Ong SJM, Hor JH, Agrawal I, Hoon S, Arogundade OA, Rodriguez MJ, Lim SM, Kim SH, Ravits J, Ng SY, Wenk MR, Lee EB, Tucker-Kellogg G, Ling SC
The Journal of cell biology 2021 Sep 6;220(9)

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Li X, Zhang J, Li D, He C, He K, Xue T, Wan L, Zhang C, Liu Q
Neuron 2021 Mar 17;109(6):957-970.e8

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Wang W, Hu X, Xia Z, Liu Z, Zhong Z, Lu Z, Liu A, Ye S, Cao Q, Wang Y, Zhu F, Ye Q
Oxidative medicine and cellular longevity 2020;2020:5849794

Activation of Mitochondrial Unfolded Protein Response in SHSY5Y Expressing APP Cells and APP/PS1 Mice.

Shen Y, Ding M, Xie Z, Liu X, Yang H, Jin S, Xu S, Zhu Z, Wang Y, Wang D, Xu L, Zhou X, Wang P, Bi J
Frontiers in cellular neuroscience 2019;13:568

OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway.

Zhang C, Zhang H, Zhang M, Lin C, Wang H, Yao J, Wei Q, Lu Y, Chen Z, Xing G, Cao X
Experimental cell research 2019 Oct 15;383(2):111512

Deletion of OSBPL2 in auditory cells increases cholesterol biosynthesis and drives reactive oxygen species production by inhibiting AMPK activity.

Wang H, Lin C, Yao J, Shi H, Zhang C, Wei Q, Lu Y, Chen Z, Xing G, Cao X
Cell death & disease 2019 Aug 19;10(9):627

The Age-dependent Elevation of miR-335-3p Leads to Reduced Cholesterol and Impaired Memory in Brain.

Raihan O, Brishti A, Molla MR, Li W, Zhang Q, Xu P, Khan MI, Zhang J, Liu Q
Neuroscience 2018 Oct 15;390:160-173

Potent effects of dioscin against obesity in mice.

Liu M, Xu L, Yin L, Qi Y, Xu Y, Han X, Zhao Y, Sun H, Yao J, Lin Y, Liu K, Peng J
Scientific reports 2015 Jan 22;5:7973

Protective effects of dioscin against alcohol-induced liver injury.

Xu T, Zheng L, Xu L, Yin L, Qi Y, Xu Y, Han X, Peng J
Archives of toxicology 2014 Mar;88(3):739-53

The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis.

Fryer LG, Jones B, Duncan EJ, Hutchison CE, Ozkan T, Williams PA, Alder O, Nieuwdorp M, Townley AK, Mensenkamp AR, Stephens DJ, Dallinga-Thie GM, Shoulders CC
The Journal of biological chemistry 2014 Feb 14;289(7):4244-61

Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.

Dong D, Qi Y, Xu L, Yin L, Xu Y, Han X, Zhao Y, Peng J
Food & function 2014 Dec;5(12):3065-3075

Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression.

Saraon P, Cretu D, Musrap N, Karagiannis GS, Batruch I, Drabovich AP, van der Kwast T, Mizokami A, Morrissey C, Jarvi K, Diamandis EP
Molecular & cellular proteomics : MCP 2013 Jun;12(6):1589-601

Identification of proteins that mediate the pro-viral functions of the interferon stimulated gene 15 in hepatitis C virus replication.

Real CI, Megger DA, Sitek B, Jahn-Hofmann K, Ickenstein LM, John MJ, Walker A, Timm J, Kuhlmann K, Eisenacher M, Meyer HE, Gerken G, Broering R, Schlaak JF
Antiviral research 2013 Dec;100(3):654-61

Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced non-alcoholic fatty liver disease in rats.

Zhang S, Zheng L, Dong D, Xu L, Yin L, Qi Y, Han X, Lin Y, Liu K, Peng J
Food chemistry 2013 Dec 1;141(3):2108-16

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: A431 cells were subjected to SDS PAGE followed by western blot with 17643-1-AP(HMGCS1 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using HMGCS1 antibody 17643-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line