17643-1-AP

antibody from Proteintech Group

Targeting: HMGCS1 HMGCS

Provider product page for 17643-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [62]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 17643-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#17643-1-AP, RRID:AB_2248359
Product name: HMGCS1 antibody
Antibody type: Polyclonal
Description: KD/KO validated HMGCS1 antibody (Cat. #17643-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

HMGCS1 protein structure - 17643-1-AP shown in red.

Submitted references ACLP promotes hypertrophic scar pathogenesis by enhancing myofibroblast activation and cholesterol synthesis-related gene expression.

Shi Y, Song Y, Wang T, Jiao Y, Dang J, Zhang Y, Xiao S, Song B, Yu Z
Pathology, research and practice 2026 Jan;277:156311

Proteome-wide C-degron activity profiling connects conditional regulation of the CTLH E3 ligase complex to ribosome biogenesis.

Grant DW, Tan S, Ramage DE, Li MZ, Di Y, Tchasovnikarova IA, Weekes MP, Elledge SJ, Warren AJ, Timms RT
bioRxiv : the preprint server for biology 2026 Jan 14;

Adaptaquin is selectively toxic to glioma stem cells through disruption of iron and cholesterol metabolism.

Vaquié AM, Shah DR, Brechbühl EES, McNicholas M, Xu Z, Stockley JH, Morcom L, Gold Diaz D, Girdler GC, Seear RV, Balmus G, Ratan RR, Bulstrode H, Nathan JA, Pathania M, Brindle KM, Rowitch DH
Molecular oncology 2026 Feb;20(2):307-330

FOXN3 integrates the KU70/KU80/SREBP-1 complex to regulate lipid metabolism in non-alcoholic fatty liver disease.

Du J, Jin L, Wang S, Hu X, Yu J, Yu J, Huo Q, Wu N, Liu X, Yang Y, Zhang Y, Huang S, Zhou J, Zheng SG, Zheng C, Zhu X
Nucleic acids research 2026 Feb 24;54(5)

Hyodeoxycholic Acid Ameliorates Metabolic Syndrome Through Pathways of Primary Bile Acid Synthesis and Fatty Acid Degradation: Insights From a Rat Model.

Chen M, Huang K, Luo W, Zhang F, Gan H, Yang Z
Drug design, development and therapy 2025;19:3611-3630

Design of PROTACs utilizing the E3 ligase GID4 for targeted protein degradation.

Li Y, Bao K, Sun J, Ge R, Zhang Q, Zhang B, Yan X, Li J, Shi F, Zhang M, Zang J, Liu M, Zhou J, Mi W, Xie S, Chen D, Shi L, Dong C
Nature structural & molecular biology 2025 Sep;32(9):1825-1837

SP600125 decreases cAMP/PKA-dependent steroid production through ATF4/DDIT3 activation in MA-10 Leydig cells.

Basque A, Izichkis LS, Martin LJ
Molecular and cellular endocrinology 2025 Sep 15;607:112613

RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.

Yang M, Cheng S, Gu H, Zhong S, Zhang D, He X, Chen W, Deng H, Ren J, Chen P, Tan M, Zhang H, Li F, Zhang Z, Hu Y, Chen J
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Oct;12(38):e01373

eIF4E Enriched Extracellular Vesicles Induce Immunosuppressive Macrophages through HMGCR-Mediated Metabolic Rewiring.

Mittal S, Nenwani M, Pulikkal Kadamberi I, Kumar S, Animasahun O, George J, Tsaih SW, Gupta P, Singh M, Geethadevi A, Dey C, Meurs N, Shankaran A, Chaluvally Raghavan P, Nagrath D, Pradeep S
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Nov;12(42):e06307

The Wnt/APC destruction complex targets SREBP2 in a β-catenin-independent pathway to control cholesterol metabolism.

Rattani A, Anderson C, Trim WV, Garita E, Imada S, Khawaled S, Shin H, Zhang J, Yilmaz ÖH, Kirschner M
bioRxiv : the preprint server for biology 2025 Nov 18;

The ErChen Decoction and Its Active Compounds Ameliorate Non-Alcoholic Fatty Liver Disease Through Activation of the AMPK Signaling Pathway.

Wang Y, Liang Y, Cheung MH, Wang X, Mo H, Gan J, Yang W, Guo J, Liang C
Pharmaceuticals (Basel, Switzerland) 2025 Nov 11;18(11)

CHD1 loss reprograms SREBP2-driven cholesterol synthesis to fuel androgen-responsive growth and castration resistance in SPOP-mutated prostate tumors.

Chen F, Li H, Wang Y, Tang X, Lin K, Li Q, Meng C, Shi W, Leo J, Liang X, Zhang J, Van V, Mahmud I, Wei B, Lorenzi PL, Raso MG, Aparicio A, Lu Y, Frigo DE, Gan B, Zhao D
Nature cancer 2025 May;6(5):854-873

m6A-induced DEAD-box RNA helicase 21 enhances lipid metabolism via 3‑hydroxy-3-methylglutaryl-CoA synthases 1 in colorectal cancer.

Gao H, Zheng S, Liang J, Wang Y, Chen L, Li H, Chen Y, Zhang F, Shi H, Han A
Translational oncology 2025 May;55:102373

Toosendanin Induces Cell Cycle Arrest and Apoptosis to Suppress Diffuse Large B-Cell Lymphoma Growth by Inhibiting PI3Kα/β and PLK1 Signaling.

Hu Q, Wang M, Chen M, Wang J, Niu T
Phytotherapy research : PTR 2025 May;39(5):1930-1945

FAM136A depletion induces mitochondrial stress and reduces mitochondrial membrane potential and ATP production.

Otsuka Y, Yano M
FEBS open bio 2025 May;15(5):738-753

Mitochondrial Genome-Encoded lncND5 Regulates Mitophagy in Hypoxic Pulmonary Artery Smooth Muscle Cell.

Bai J, He S, Wang X, Zhang L, Ma C, Gao D, Yuan H, Mei J, Guan X, Yu H, Wan K, Zhu D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 May 31;39(10):e70618

Decreased protein activator of interferon induced protein kinase (PRKRA) involved in menopause-related cholesterol metabolic disorders by regulating cholesterol biosynthesis.

Xu L, Xu J, Shi W, Zhang S, Guo T, Zou S
Lipids in health and disease 2025 May 10;24(1):171

High glucose levels promote glycolysis and cholesterol synthesis via ERRα and suppress the autophagy-lysosomal pathway in endometrial cancer.

Mao X, Huang L, Liu X, Lin X, Wu Q, Wang X, Ren Y, Ma J, Zhang M, Lin Y, Ralser DJ, Mustea A, Chen G, Sun P
Cell death & disease 2025 Mar 17;16(1):182

DHODH Blockade Induces Ferroptosis in Neuroblastoma by Modulating the Mevalonate Pathway.

Shir JC, Chen PY, Kuo CH, Hsieh CH, Chang HY, Lee HC, Huang CH, Hsu CH, Hsu WM, Huang HC, Juan HF
Molecular & cellular proteomics : MCP 2025 Jul;24(7):101014

Glutamine-driven metabolic reprogramming promotes CAR-T cell function through mTOR-SREBP2 mediated HMGCS1 upregulation in ovarian cancer.

Chen J, Zhao L, Li W, Wang S, Li J, Lv Z, Zhao Y, Liang J, Hu Z, Pan F, He L, Gu L, Guo Z
Journal of translational medicine 2025 Jul 17;23(1):803

Fatty Acid Esterification of Octacosanol Attenuates Triglyceride and Cholesterol Synthesis in Mice.

Kamchonemenukool S, Koh YC, Ho PY, Pan MH, Weerawatanakorn M
Journal of agricultural and food chemistry 2025 Jan 29;73(4):2430-2442

A dietary pan-amino acid dropout screen in vivo reveals a critical role for histidine in T-ALL.

Mandleywala K, Ulrich S, da Silva-Diz V, Sharma P, Thai C, Kim O, Aleksandrova M, Chung G, Eggers C, Lütjohann D, Kulkarni T, Singh A, Elena Díaz-Rubio M, Wierer M, Leidel SA, Su X, White EP, Rabinowitz JD, Morscher RJ, Herranz D
bioRxiv : the preprint server for biology 2025 Dec 23;

Collateral metabolic vulnerabilities unveiled by loss of isozyme diversity in breast cancer.

Ding R, Yu TJ, Jiang YZ, Xiao Y, Shao ZM
Genome medicine 2025 Dec 12;18(1):7

Inhibition of Mettl3-mediated m6A RNA modification of HMGCS1 protects retinal ganglion cells from glutamate excitotoxicity-induced ferroptosis in a rat model of glaucoma.

Wang C, Feng L, Fang W, Zhang C, Zhang W, Zhu W, He Y, Xia Z, Song W, Xia X
International journal of surgery (London, England) 2025 Dec 1;111(12):9147-9165

PKMYT1 knockdown inhibits cholesterol biosynthesis and promotes the drug sensitivity of triple-negative breast cancer cells to atorvastatin.

Gao W, Guo X, Sun L, Gai J, Cao Y, Zhang S
PeerJ 2024;12:e17749

Ethanol Extract of Propolis Attenuates Liver Lipid Metabolism Disorder in High-Fat Diet-Fed SAMP8 Mice.

Xie QY, Shu X, Liu T, Chen LL, Luo LP
Molecular nutrition & food research 2024 Sep;68(17):e2400297

Gypenoside L inhibits hepatocellular carcinoma by targeting the SREBP2-HMGCS1 axis and enhancing immune response.

Xiao MY, Pei WJ, Li S, Li FF, Xie P, Luo HT, Hyun Yoo H, Piao XL
Bioorganic chemistry 2024 Sep;150:107539

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway.

Yi SA, Sepic S, Schulman BA, Ordureau A, An H
Molecular cell 2024 Jun 6;84(11):2166-2184.e9

Treg and neutrophil extracellular trap interaction contributes to the development of immunosuppression in sepsis.

Shi Y, Wu D, Wang Y, Shao Y, Zeng F, Zhou D, Zhang H, Miao C
JCI insight 2024 Jun 18;9(14)

Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism.

Zhao L, Qiu Z, Yang Z, Xu L, Pearce TM, Wu Q, Yang K, Li F, Saulnier O, Fei F, Yu H, Gimple RC, Varadharajan V, Liu J, Hendrikse LD, Fong V, Wang W, Zhang J, Lv D, Lee D, Lehrich BM, Jin C, Ouyang L, Dixit D, Wu H, Wang X, Sloan AE, Wang X, Huan T, Mark Brown J, Goldman SA, Taylor MD, Zhou S, Rich JN
Nature cancer 2024 Jan;5(1):147-166

Hypoxia upregulating ACSS2 enhances lipid metabolism reprogramming through HMGCS1 mediated PI3K/AKT/mTOR pathway to promote the progression of pancreatic neuroendocrine neoplasms.

Gu D, Ye M, Zhu G, Bai J, Chen J, Yan L, Yu P, Lu F, Hu C, Zhong Y, Liu P, He Q, Tang Q
Journal of translational medicine 2024 Jan 23;22(1):93

CSN6-SPOP-HMGCS1 Axis Promotes Hepatocellular Carcinoma Progression via YAP1 Activation.

Li K, Zhang J, Lyu H, Yang J, Wei W, Wang Y, Luo H, Zhang Y, Jiang X, Yi H, Wang M, Zhang C, Wu K, Xiao L, Wen W, Xu H, Li G, Wan Y, Yang F, Yang R, Fu X, Qin B, Zhou Z, Zhang H, Lee MH
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Apr;11(14):e2306827

Complexes of Soluble Dietary Fiber and Polyphenols from Lotus Root Regulate High-Fat Diet-Induced Hyperlipidemia in Mice.

Zheng Z, Gao W, Zhu Z, Li S, Chen X, Cravotto G, Sui Y, Zhou L
Antioxidants (Basel, Switzerland) 2024 Apr 16;13(4)

CCR9 overexpression promotes T-ALL progression by enhancing cholesterol biosynthesis.

Jamal M, Lei Y, He H, Zeng X, Bangash HI, Xiao D, Shao L, Zhou F, Zhang Q
Frontiers in pharmacology 2023;14:1257289

miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway.

Xing K, Bian X, Shi D, Dong S, Zhou H, Xiao S, Bai J, Wu W
Journal of hepatocellular carcinoma 2023;10:2173-2185

Gypenosides suppress hepatocellular carcinoma cells by blocking cholesterol biosynthesis through inhibition of MVA pathway enzyme HMGCS1.

Xiao MY, Li FF, Xie P, Qi YS, Xie JB, Pei WJ, Luo HT, Guo M, Gu YL, Piao XL
Chemico-biological interactions 2023 Sep 25;383:110674

Morusinol Extracted from Morus alba Inhibits Cell Proliferation and Induces Autophagy via FOXO3a Nuclear Accumulation-Mediated Cholesterol Biosynthesis Obstruction in Colorectal Cancer.

Zhang X, Dong Z, Yang Y, Liu C, Li J, Sun W, Zhu Y, Shen Y, Wang Z, Lü M, Cui H
Journal of agricultural and food chemistry 2023 Nov 1;71(43):16016-16031

P4HA1 activates HMGCS1 to promote nasopharyngeal carcinoma ferroptosis resistance and progression.

Zhou R, Qiu L, Zhou L, Geng R, Yang S, Wu J
Cellular signalling 2023 May;105:110609

ERRα Up-Regulates Invadopodia Formation by Targeting HMGCS1 to Promote Endometrial Cancer Invasion and Metastasis.

Tang S, Ma J, Su P, Lei H, Tong Y, Cai L, Xu S, Mao X, Sun P
International journal of molecular sciences 2023 Feb 16;24(4)

Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis.

Zhou W, Liu H, Yuan Z, Zundell J, Towers M, Lin J, Lombardi S, Nie H, Murphy B, Yang T, Wang C, Liao L, Goldman AR, Kannan T, Kossenkov AV, Drapkin R, Montaner LJ, Claiborne DT, Zhang N, Wu S, Zhang R
Cancer cell 2023 Apr 10;41(4):740-756.e10

MiR-122-5p regulates the mevalonate pathway by targeting p53 in non-small cell lung cancer.

Zheng YK, Zhou ZS, Wang GZ, Tu JY, Cheng HB, Ma SZ, Ke C, Wang Y, Jian QP, Shu YH, Wu XW
Cell death & disease 2023 Apr 1;14(4):234

Phyllanthus Niruri L. Exerts Protective Effects Against the Calcium Oxalate-Induced Renal Injury via Ellgic Acid.

Li MT, Liu LL, Zhou Q, Huang LX, Shi YX, Hou JB, Lu HT, Yu B, Chen W, Guo ZY
Frontiers in pharmacology 2022;13:891788

Itraconazole Inhibits the Growth of Cutaneous Squamous Cell Carcinoma by Targeting HMGCS1/ACSL4 Axis.

Xu C, Zhuo Y, Liu Y, Chen H
Frontiers in pharmacology 2022;13:828983

Upregulation of Superenhancer-Driven LncRNA FASRL by USF1 Promotes De Novo Fatty Acid Biosynthesis to Exacerbate Hepatocellular Carcinoma.

Peng JY, Cai DK, Zeng RL, Zhang CY, Li GC, Chen SF, Yuan XQ, Peng L
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Oct 28;10(1):e2204711

Discovery and Application of Postnatal Nucleus Pulposus Progenitors Essential for Intervertebral Disc Homeostasis and Degeneration.

Gao B, Jiang B, Xing W, Xie Z, Luo Z, Zou W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 May;9(13):e2104888

Krüppel-like Factor 13 Promotes HCC Progression by Transcriptional Regulation of HMGCS1-mediated Cholesterol Synthesis.

Chen CC, Xie XM, Zhao XK, Zuo S, Li HY
Journal of clinical and translational hepatology 2022 Dec 28;10(6):1125-1137

miRNA-223 Suppresses Mouse Gallstone Formation by Targeting Key Transporters in Hepatobiliary Cholesterol Secretion Pathway.

Zhao F, Ma S, Zhou Y, Wei B, Hao Z, Cui X, Xing L, Liu G, Jin L, Ma T, Shi L
International journal of biological sciences 2021;17(15):4459-4473

Transcriptomic Analysis Reveals the Protective Effects of Empagliflozin on Lipid Metabolism in Nonalcoholic Fatty Liver Disease.

Ma Y, Kan C, Qiu H, Liu Y, Hou N, Han F, Shi J, Sun X
Frontiers in pharmacology 2021;12:793586

TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.

Ho WY, Chang JC, Lim K, Cazenave-Gassiot A, Nguyen AT, Foo JC, Muralidharan S, Viera-Ortiz A, Ong SJM, Hor JH, Agrawal I, Hoon S, Arogundade OA, Rodriguez MJ, Lim SM, Kim SH, Ravits J, Ng SY, Wenk MR, Lee EB, Tucker-Kellogg G, Ling SC
The Journal of cell biology 2021 Sep 6;220(9)

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Li X, Zhang J, Li D, He C, He K, Xue T, Wan L, Zhang C, Liu Q
Neuron 2021 Mar 17;109(6):957-970.e8

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Wang W, Hu X, Xia Z, Liu Z, Zhong Z, Lu Z, Liu A, Ye S, Cao Q, Wang Y, Zhu F, Ye Q
Oxidative medicine and cellular longevity 2020;2020:5849794

Activation of Mitochondrial Unfolded Protein Response in SHSY5Y Expressing APP Cells and APP/PS1 Mice.

Shen Y, Ding M, Xie Z, Liu X, Yang H, Jin S, Xu S, Zhu Z, Wang Y, Wang D, Xu L, Zhou X, Wang P, Bi J
Frontiers in cellular neuroscience 2019;13:568

OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway.

Zhang C, Zhang H, Zhang M, Lin C, Wang H, Yao J, Wei Q, Lu Y, Chen Z, Xing G, Cao X
Experimental cell research 2019 Oct 15;383(2):111512

Deletion of OSBPL2 in auditory cells increases cholesterol biosynthesis and drives reactive oxygen species production by inhibiting AMPK activity.

Wang H, Lin C, Yao J, Shi H, Zhang C, Wei Q, Lu Y, Chen Z, Xing G, Cao X
Cell death & disease 2019 Aug 19;10(9):627

The Age-dependent Elevation of miR-335-3p Leads to Reduced Cholesterol and Impaired Memory in Brain.

Raihan O, Brishti A, Molla MR, Li W, Zhang Q, Xu P, Khan MI, Zhang J, Liu Q
Neuroscience 2018 Oct 15;390:160-173

Potent effects of dioscin against obesity in mice.

Liu M, Xu L, Yin L, Qi Y, Xu Y, Han X, Zhao Y, Sun H, Yao J, Lin Y, Liu K, Peng J
Scientific reports 2015 Jan 22;5:7973

Protective effects of dioscin against alcohol-induced liver injury.

Xu T, Zheng L, Xu L, Yin L, Qi Y, Xu Y, Han X, Peng J
Archives of toxicology 2014 Mar;88(3):739-53

The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis.

Fryer LG, Jones B, Duncan EJ, Hutchison CE, Ozkan T, Williams PA, Alder O, Nieuwdorp M, Townley AK, Mensenkamp AR, Stephens DJ, Dallinga-Thie GM, Shoulders CC
The Journal of biological chemistry 2014 Feb 14;289(7):4244-61

Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.

Dong D, Qi Y, Xu L, Yin L, Xu Y, Han X, Zhao Y, Peng J
Food & function 2014 Dec;5(12):3065-3075

Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression.

Saraon P, Cretu D, Musrap N, Karagiannis GS, Batruch I, Drabovich AP, van der Kwast T, Mizokami A, Morrissey C, Jarvi K, Diamandis EP
Molecular & cellular proteomics : MCP 2013 Jun;12(6):1589-601

Identification of proteins that mediate the pro-viral functions of the interferon stimulated gene 15 in hepatitis C virus replication.

Real CI, Megger DA, Sitek B, Jahn-Hofmann K, Ickenstein LM, John MJ, Walker A, Timm J, Kuhlmann K, Eisenacher M, Meyer HE, Gerken G, Broering R, Schlaak JF
Antiviral research 2013 Dec;100(3):654-61

Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced non-alcoholic fatty liver disease in rats.

Zhang S, Zheng L, Dong D, Xu L, Yin L, Qi Y, Han X, Lin Y, Liu K, Peng J
Food chemistry 2013 Dec 1;141(3):2108-16

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: A431 cells were subjected to SDS PAGE followed by western blot with 17643-1-AP(HMGCS1 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using HMGCS1 antibody 17643-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line