GTX112346

antibody from GeneTex

Targeting: HMGCS1 HMGCS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX112346

Provider

GeneTex

Proper citation

Product name

HMGCS1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7.5% SDS PAGE GTX112346 diluted at 1:5000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ?g of whole cell lysate) A: mouse liver 7.5% SDS PAGE GTX112346 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGCS1 antibody detects HMGCS1 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extract7.5 % SDS-PAGEHMGCS1 antibody (GTX112346) dilution: 1:5000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGCS1 antibody detects HMGCS1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with HMGCS1 antibody (GTX112346) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGCS1 antibody detects HMGCS1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGCS1 protein stained by HMGCS1 antibody (GTX112346) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGCS1 antibody detects HMGCS1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HMGCS1 antibody (GTX112346) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGCS1 antibody detects HMGCS1 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HMGCS1 antibody (GTX112346) dilution: 1:500.