Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF717 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CCL28 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CCL28 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CCL28 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A role for CCL28-CCR3 in T-cell homing to the human upper airway mucosa.
CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
Danilova E, Skrindo I, Gran E, Hales BJ, Smith WA, Jahnsen J, Johansen FE, Jahnsen FL, Baekkevold ES
Mucosal immunology 2015 Jan;8(1):107-14
Mucosal immunology 2015 Jan;8(1):107-14
CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1452-61
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1452-61
CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1452-61
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1452-61
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody. Recombinant Human CCL28 (Catalog # 717-VC) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL28 (4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF717). The ND50 is typically 40-100 µg/mL.