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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB717 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL28 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCL28 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 19, 21, 22, 25, or recombinant rat CCL20 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9NRJ3- Isotype
- IgG
- Antibody clone number
- 62705
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
Epithelial inflammation is associated with CCL28 production and the recruitment of regulatory T cells expressing CCR10.
Epithelial inflammation is associated with CCL28 production and the recruitment of regulatory T cells expressing CCR10.
CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
Nature 2011 Jul 13;475(7355):226-30
Nature 2011 Jul 13;475(7355):226-30
Epithelial inflammation is associated with CCL28 production and the recruitment of regulatory T cells expressing CCR10.
Eksteen B, Miles A, Curbishley SM, Tselepis C, Grant AJ, Walker LS, Adams DH
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):593-603
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):593-603
Epithelial inflammation is associated with CCL28 production and the recruitment of regulatory T cells expressing CCR10.
Eksteen B, Miles A, Curbishley SM, Tselepis C, Grant AJ, Walker LS, Adams DH
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):593-603
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):593-603
CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1452-61
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1452-61
CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1452-61
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1452-61
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody. Recombinant Human CCL28 (Catalog # 717-VC) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL28 (4 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL28 Monoclonal Antibody (Catalog # MAB717). The ND50 is typically 15-45 µg/mL.
