PA5-120413

antibody from Invitrogen Antibodies

Targeting: FREM2 DKFZp686J0811

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120413

Provider

Invitrogen Antibodies

Product name

FREM2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: Jurkat, HepG2, 293T. The target is usually found in the following locations: Cell membrane, Extracellular side, Single-pass type I membrane protein. Immunogen sequence: QPAAFGRALL SPGLAGAAGV PAEEAIVLAN RGLRVPFGRE VWLDPLHDLV LQVQPGDRCA VSVLDNDALA QRPGRLSPKR FPCDFGPGEV RYSHLGARSP SRDRVRLQLR YDAPGGAVVL PLVLEVEVVF TQLEVVTRNL PLVVEELLGT SNALDARSLE FAFQPETEEC RVGILSGLGA LPRYGELLHY PQVPGGAREG GAPETLLMDC KAFQELGVRY RHTAASRSPN RDWIPMVVEL RSRGAPVGSP ALKR

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.43 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of FREM2 in various cell lines using a FREM2 Polyclonal antibody (Product # PA5-120413) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 150s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of FREM2 in NIH/3T3 cells using a FREM2 Polyclonal antibody (Product # PA5-120413). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of FREM2 in NIH/3T3 cells using a FREM2 Polyclonal antibody (Product # PA5-120413). Blue: DAPI for nuclear staining.