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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX104102 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104102, RRID:AB_1950497
- Product name
- BRAF35 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ug whole cell lysate)A: 293T12% SDS PAGEGTX104102 diluted at 1:500
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BRAF35 antibody detects BRAF35 protein at nucleus by immunofluorescent analysis.Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BRAF35 protein stained by BRAF35 antibody (GTX104102) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BRAF35 antibody immunoprecipitates BRAF35 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 £gg 293T whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of BRAF35 protein by 2 £gg of BRAF35 antibody (GTX104102) 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 antibody (GTX104102) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
