Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX115399 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115399, RRID:AB_10620410
- Product name
- BRAF35 antibody [N2C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 10% SDS PAGE GTX115399 diluted at 1:2000
Supportive validation
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- GeneTex (provider)
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- Experimental details
- BRAF35 antibody immunoprecipitates BRAF35 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 3 £gg of pre-immune rabbit IgG B : Immunoprecipitation of BRAF35 by 3 £gg of BRAF35 antibody (GTX115399) 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 antibody (GTX115399) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using HMG20B(GTX115399) antibody at 1:500 dilution.