H00010362-M01
antibody from Novus Biologicals
Targeting: HMG20B
BRAF25, BRAF35, HMGX2, HMGXB2, SMARCE1r, SOXL
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00010362-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010362-M01, RRID:AB_537200
- Product name
- Mouse Monoclonal BRAF35 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. HMG20B - high-mobility group 20B
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The E3 ubiquitin ligase MID1/TRIM18 promotes atypical ubiquitination of the BRCA2-associated factor 35, BRAF35.
A cancer-associated mutation inactivates a region of the high-mobility group protein HMG20b essential for cytokinesis.
Control of neuronal differentiation by sumoylation of BRAF35, a subunit of the LSD1-CoREST histone demethylase complex.
Zanchetta ME, Napolitano LMR, Maddalo D, Meroni G
Biochimica et biophysica acta. Molecular cell research 2017 Oct;1864(10):1844-1854
Biochimica et biophysica acta. Molecular cell research 2017 Oct;1864(10):1844-1854
A cancer-associated mutation inactivates a region of the high-mobility group protein HMG20b essential for cytokinesis.
Lee M, Venkitaraman AR
Cell cycle (Georgetown, Tex.) 2014;13(16):2554-63
Cell cycle (Georgetown, Tex.) 2014;13(16):2554-63
Control of neuronal differentiation by sumoylation of BRAF35, a subunit of the LSD1-CoREST histone demethylase complex.
Ceballos-Chávez M, Rivero S, García-Gutiérrez P, Rodríguez-Paredes M, García-Domínguez M, Bhattacharya S, Reyes JC
Proceedings of the National Academy of Sciences of the United States of America 2012 May 22;109(21):8085-90
Proceedings of the National Academy of Sciences of the United States of America 2012 May 22;109(21):8085-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRAF35 Antibody (1F6) [H00010362-M01] - Analysis of HMG20B in extracts from 293T, HeLa and A549 cell using anti-HMG20B monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRAF35 Antibody (1F6) [H00010362-M01] - HMG20B monoclonal antibody (M01), clone IF6 Analysis of HMG20B expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: BRAF35 Antibody (1F6) [H00010362-M01] - Analysis of monoclonal antibody to HMG20B on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRAF35 Antibody (1F6) [H00010362-M01] - Analysis of monoclonal antibody to HMG20B on formalin-fixed paraffin-embedded human lung cancer. Antibody concentration 1.8 ug/ml.