Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-63525 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM52B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QLPSSLDTLP GYEEALHMSR FTVAMCGQKA PDLPPVPEEK QLPPTEKEST RIVDSWN Highest antigen sequence identity to the following orthologs: Mouse - 75%, Rat - 77%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TMEM52B in control (vector only transfected HEK293T lysate) and TMEM52B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a TMEM52B Polyclonal Antibody (Product # PA5-63525).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of TMEM52B in human cell line CACO-2 using a TMEM52B Polyclonal Antibody (Product # PA5-63525) shows localization to focal adhesion sites.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TMEM52B in human kidney tissue shows strong membranous positivity in a subset of distal tubules. Samples were probed using a TMEM52B Polyclonal Antibody (Product # PA5-63525).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TMEM52B in human kidney and pancreas tissues using TMEM52B Polyclonal Antibody (Product # PA5-63525). Corresponding TMEM52B RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TMEM52B in human pancreas using TMEM52B Polyclonal Antibody (Product # PA5-63525) shows low expression as expected.