12024-1-AP

antibody from Proteintech Group

Targeting: NUSAP1 ANKT, BM037, FLJ13421, LNP, PRO0310p1, Q0310, SAPL

Provider product page for 12024-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [40]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 12024-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12024-1-AP, RRID:AB_2157789
Product name: NUSAP1 antibody
Antibody type: Polyclonal
Description: KD/KO validated NUSAP1 antibody (Cat. #12024-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NUSAP1 protein structure - 12024-1-AP shown in red.

Submitted references Immunotherapeutic value of NUSAP1 associated with bladder cancer through a comprehensive analysis of 33 human cancer cases.

Wen X, Hou J, Chu Y, Liao G, Wu G, Fang S, Xiao S, Qiu L, Xiong L
American journal of cancer research 2024;14(3):959-978

Unveiling NUSAP1 as a common gene signature linking chronic HBV infection and HBV-related HCC.

Meng J, Yang Z, Jiang X, Zou J
Discover oncology 2024 Mar 5;15(1):61

NUSAP1 promotes pancreatic ductal adenocarcinoma progression by drives the epithelial-mesenchymal transition and reduces AMPK phosphorylation.

Liu Y, Tang R, Meng QC, Shi S, Xu J, Yu XJ, Zhang B, Wang W
BMC cancer 2024 Jan 16;24(1):87

Comprehensive pan-cancer analysis reveals NUSAP1 is a novel predictive biomarker for prognosis and immunotherapy response.

Zheng H, Wang M, Zhang S, Hu D, Yang Q, Chen M, Zhang X, Zhang Y, Dai J, Liou YC
International journal of biological sciences 2023;19(14):4689-4708

Expression Profile and Gene Regulation Network of NUSAP1 in Pan Cancers Based on Integrated Bioinformatics Analysis.

Zhu X, Wu Y, Liao L, Huang W, Yuan L, Huang J, Zhan Y, Liu L
International journal of general medicine 2023;16:4235-4248

Bioinformatics-based screening of key genes for transformation of tyrosine kinase inhibitor-resistant lung adenocarcinoma to small cell lung cancer.

Zhang Y, Chen Q, Huang T, Zhu D, Lu Y
Frontiers in medicine 2023;10:1203461

LINC01393, a Novel Long Non-Coding RNA, Promotes the Cell Proliferation, Migration and Invasion through MiR-128-3p/NUSAP1 Axis in Glioblastoma.

Li D, Hu J, Li S, Zhou C, Feng M, Li L, Gao Y, Chen X, Wu X, Cao Y, Hao B, Chen L
International journal of molecular sciences 2023 Mar 20;24(6)

NUSAP1 regulates mouse oocyte meiotic maturation.

Yu L, Kong N, Lin Y, Qiu P, Xu Q, Zhang Y, Zhen X, Yan G, Sun H, Mei J, Cao G
Journal of cellular biochemistry 2023 Dec;124(12):1931-1947

Non-canonical function of histone methyltransferase G9a in the translational regulation of chronic inflammation.

Muneer A, Wang L, Xie L, Zhang F, Wu B, Mei L, Lenarcic EM, Feng EH, Song J, Xiong Y, Yu X, Wang C, Jain K, Strahl BD, Cook JG, Wan YY, Moorman NJ, Song H, Jin J, Chen X
Cell chemical biology 2023 Dec 21;30(12):1525-1541.e7

FoxM1 coordinates cell division, protein synthesis, and mitochondrial activity in a subset of β cells during acute metabolic stress.

Kobiita A, Silva PN, Schmid MW, Stoffel M
Cell reports 2023 Aug 29;42(8):112986

NUSAP1 Could be a Potential Target for Preventing NAFLD Progression to Liver Cancer.

Zeng T, Chen G, Qiao X, Chen H, Sun L, Ma Q, Li N, Wang J, Dai C, Xu F
Frontiers in pharmacology 2022;13:823140

Knockdown of NUSAP1 inhibits cell proliferation and invasion through downregulation of TOP2A in human glioblastoma.

Hu Y, Xue Z, Qiu C, Feng Z, Qi Q, Wang J, Jin W, Zhong Z, Liu X, Li W, Zhang Q, Huang B, Chen A, Wang J, Yang N, Zhou W
Cell cycle (Georgetown, Tex.) 2022 Sep;21(17):1842-1855

The mechanism of acentrosomal spindle assembly in human oocytes.

Wu T, Dong J, Fu J, Kuang Y, Chen B, Gu H, Luo Y, Gu R, Zhang M, Li W, Dong X, Sun X, Sang Q, Wang L
Science (New York, N.Y.) 2022 Nov 18;378(6621):eabq7361

Identification and clinical validation of NUSAP1 as a novel prognostic biomarker in ovarian cancer.

Gou R, Zheng M, Hu Y, Gao L, Wang S, Liu O, Li X, Zhu L, Liu J, Lin B
BMC cancer 2022 Jun 23;22(1):690

Nucleolar and spindle‑associated protein 1 promotes non‑small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D.

Ling B, Wei P, Xiao J, Cen B, Wei H, Feng X, Ye G, Li S, Zhang Z, Liang W, Huang S, Huang W
Oncology reports 2021 Mar;45(3):1044-1058

AMD3465 (hexahydrobromide) rescues the MG63 osteoblasts against the apoptosis induced by high glucose.

Zhang Z, Wu H, Huang S, Chen L, Chen X, Zhang W, Liao L, Zhang X
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021 Jun;138:111476

Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-β Signaling Pathway.

Gao S, Yin H, Tong H, Zhan K, Yang G, Hossain MA, Li T, Gou X, He W
OncoTargets and therapy 2020;13:813-825

NUSAP1 Promotes Gastric Cancer Tumorigenesis and Progression by Stabilizing the YAP1 Protein.

Guo H, Zou J, Zhou L, Zhong M, He Y, Huang S, Chen J, Li J, Xiong J, Fang Z, Xiang X
Frontiers in oncology 2020;10:591698

RepoMan stimulates the chromosome-dependent pathway of microtubule assembly.

De Munter S, Van der Hoeven G, Bollen M
Cell cycle (Georgetown, Tex.) 2020 Nov;19(22):3029-3041

Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics.

Wang L, Muneer A, Xie L, Zhang F, Wu B, Mei L, Lenarcic EM, Feng EH, Song J, Xiong Y, Yu X, Wang C, Gheorghe C, Torralba K, Cook JG, Wan YY, Moorman NJ, Song H, Jin J, Chen X
bioRxiv : the preprint server for biology 2020 Nov 16;

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

Wang Y, Song W, Wang J, Wang T, Xiong X, Qi Z, Fu W, Yang X, Chen YG
The Journal of experimental medicine 2020 Feb 3;217(2)

Knockdown of SP1/Syncytin1 axis inhibits the proliferation and metastasis through the AKT and ERK1/2 signaling pathways in non-small cell lung cancer.

Li X, Fu Y, Xia X, Zhang X, Xiao K, Zhuang X, Zhang Y
Cancer medicine 2019 Sep;8(12):5750-5759

Hepatitis B virus X protein enhances hepatocarcinogenesis by depressing the targeting of NUSAP1 mRNA by miR-18b.

Yang Z, Li J, Feng G, Wang Y, Yang G, Liu Y, Zhang S, Feng J, Zhang X
Cancer biology & medicine 2019 May;16(2):276-287

Nucleolar spindle-associated protein 1 promotes tumorigenesis and predicts poor prognosis in human esophageal squamous cell carcinoma.

Guan C, Liu Z, Lu C, Xiao M, Shi H, Ni R, Bian Z
Journal of cellular biochemistry 2019 Jul;120(7):11726-11737

Downregulation of nucleolar and spindle-associated protein 1 expression suppresses liver cancer cell function.

Wang Y, Ju L, Xiao F, Liu H, Luo X, Chen L, Lu Z, Bian Z
Experimental and therapeutic medicine 2019 Apr;17(4):2969-2978

Nucleolar and spindle-associated protein 1 is a tumor grade correlated prognosis marker for glioma patients.

Zhu T, Xie P, Gao YF, Huang MS, Li X, Zhang W, Zhou HH, Liu ZQ
CNS neuroscience & therapeutics 2018 Mar;24(3):178-186

High NUSAP1 expression predicts poor prognosis in colon cancer.

Liu Z, Guan C, Lu C, Liu Y, Ni R, Xiao M, Bian Z
Pathology, research and practice 2018 Jul;214(7):968-973

microRNA 193a-5p Regulates Levels of Nucleolar- and Spindle-Associated Protein 1 to Suppress Hepatocarcinogenesis.

Roy S, Hooiveld GJ, Seehawer M, Caruso S, Heinzmann F, Schneider AT, Frank AK, Cardenas DV, Sonntag R, Luedde M, Trautwein C, Stein I, Pikarsky E, Loosen S, Tacke F, Ringelhan M, Avsaroglu SK, Goga A, Buendia MA, Vucur M, Heikenwalder M, Zucman-Rossi J, Zender L, Roderburg C, Luedde T
Gastroenterology 2018 Dec;155(6):1951-1966.e26

NUSAP1 promotes invasion and metastasis of prostate cancer.

Gordon CA, Gong X, Ganesh D, Brooks JD
Oncotarget 2017 May 2;8(18):29935-29950

APLP2, RRM2, and PRC1: New Putative Markers for the Differential Diagnosis of Thyroid Follicular Lesions.

Castelblanco E, Zafon C, Maravall J, Gallel P, Martinez M, Capel I, Bella MR, Halperin I, Temprana J, Iglesias C, Puig-Domingo M, Robledo M, Matias-Guiu X, Mauricio D
Thyroid : official journal of the American Thyroid Association 2017 Jan;27(1):59-66

Targeting PI3K/mTOR signaling exerts potent antitumor activity in pheochromocytoma in vivo.

Lee M, Minaskan N, Wiedemann T, Irmler M, Beckers J, Yousefi BH, Kaissis G, Braren R, Laitinen I, Pellegata NS
Endocrine-related cancer 2017 Jan;24(1):1-15

Down-Regulation of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) Expression Suppresses Tumor and Cell Proliferation and Enhances Anti-Tumor Effect of Paclitaxel in Oral Squamous Cell Carcinoma.

Okamoto A, Higo M, Shiiba M, Nakashima D, Koyama T, Miyamoto I, Kasama H, Kasamatsu A, Ogawara K, Yokoe H, Tanzawa H, Uzawa K
PloS one 2015;10(11):e0142252

High Levels of Nucleolar Spindle-Associated Protein and Reduced Levels of BRCA1 Expression Predict Poor Prognosis in Triple-Negative Breast Cancer.

Chen L, Yang L, Qiao F, Hu X, Li S, Yao L, Yang XL, Shao ZM
PloS one 2015;10(10):e0140572

The selective inhibition of protein phosphatase-1 results in mitotic catastrophe and impaired tumor growth.

Winkler C, De Munter S, Van Dessel N, Lesage B, Heroes E, Boens S, Beullens M, Van Eynde A, Bollen M
Journal of cell science 2015 Dec 15;128(24):4526-37

NUSAP1 expression is upregulated by loss of RB1 in prostate cancer cells.

Gordon CA, Gulzar ZG, Brooks JD
The Prostate 2015 Apr 1;75(5):517-26

Enhanced protein degradation by branched ubiquitin chains.

Meyer HJ, Rape M
Cell 2014 May 8;157(4):910-21

Microtubule-dependent regulation of mitotic protein degradation.

Song L, Craney A, Rape M
Molecular cell 2014 Jan 23;53(2):179-92

Transcriptome analysis of MENX-associated rat pituitary adenomas identifies novel molecular mechanisms involved in the pathogenesis of human pituitary gonadotroph adenomas.

Lee M, Marinoni I, Irmler M, Psaras T, Honegger JB, Beschorner R, Anastasov N, Beckers J, Theodoropoulou M, Roncaroli F, Pellegata NS
Acta neuropathologica 2013 Jul;126(1):137-50

Increased expression of NuSAP in recurrent prostate cancer is mediated by E2F1.

Gulzar ZG, McKenney JK, Brooks JD
Oncogene 2013 Jan 3;32(1):70-7

Regulated degradation of spindle assembly factors by the anaphase-promoting complex.

Song L, Rape M
Molecular cell 2010 May 14;38(3):369-82

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: rat colon tissue were subjected to SDS PAGE followed by western blot with 12024-1-AP( NUSAP1 Antibody) at dilution of 1:1000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using NUSAP1 antibody 12024-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 12024-1-AP(NUSAP1 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue