Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AF5944 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Glyoxalase II Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat Glyoxalase II in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Glyoxalase I is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q16775
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat Glyoxalase II by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, human liver, human kidney, mouse liver, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glyoxalase II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Glyoxalase II at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.