OSR00122W

antibody from Invitrogen Antibodies

Targeting: HCN1 BCNG-1, BCNG1, HAC-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

OSR00122W

Provider

Invitrogen Antibodies

Product name

HCN1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-HCN1 Polyclonal Antibody (Product # OSR00122W) and a 110 kDa band corresponding to Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 was observed only in Mouse Brain, Rat Brain, Mouse Cerebellum and Rat cerebellum but not in any other tissues tested like Mouse Heart, Rat Heart and Mouse Skeletal Muscle. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Cerebellum (Lane 3), Rat Cerebellum (Lane 4), Mouse Heart (Lane 5), Rat Heart (Lane 6) and Mouse Skeletal Muscle (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Uncharacterized bands of ~30 kDa and ~53 kDa were also observed across the high expressing tissues.