Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-53950 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HCN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PSLPHEVSTL ISRPHPTVGE SLASIPQPVT AVPGTGLQAG GRSTVPQRVT LFRQMSSGAI PPNRGVPPAP PPPAAALPRE SSSVLNTDPD AE Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 87%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Contribution of HCN1 variant to sinus bradycardia: A case report.
Yu H, Gall B, Newman M, Hathaway Q, Brundage K, Ammer A, Mathers P, Siderovski D, Hull RW
Journal of arrhythmia 2021 Oct;37(5):1337-1347
Journal of arrhythmia 2021 Oct;37(5):1337-1347
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HCN1 in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human cerebral cortex tissue. Samples were probed using a HCN1 Polyclonal Antibody (Product # PA5-53950).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-HCN1 Polyclonal Antibody (Product # PA5-53950) and a 110 kDa band corresponding to Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 was observed only in Mouse Brain, Rat Brain, Mouse Cerebellum and Rat cerebellum but not in any other tissues tested like Mouse Heart, Rat Heart and Mouse Skeletal Muscle. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Cerebellum (Lane 3), Rat Cerebellum (Lane 4), Mouse Heart (Lane 5), Rat Heart (Lane 6) and Mouse Skeletal Muscle (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HCN1 in human cerebral cortex tissue shows moderate cytoplasmic positivity in neuronal cells. Samples were probed using a HCN1 Polyclonal Antibody (Product # PA5-53950).