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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-117981 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HCN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HCN1 Polyclonal Antibody (Product # PA5-117981) at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 99 kDa. Observed band size: 100 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HCN1 Immunoprecipitation using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate 4 µL with HCN1 Polyclonal Antibody (Product # PA5-117981) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: HCN1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 99 kDa. Observed band size: 100 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human HCN1 in human brain with HCN1 Polyclonal Antibody (Product # PA5-117981, 1:500 dilution, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HCN1 Immunoprecipitation using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate 4 µL with HCN1 Polyclonal Antibody (Product # PA5-117981) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: HCN1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 99 kDa. Observed band size: 100 kDa.
