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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-96287 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYBBP1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DLTVLLGVLQ GQQQSLQQGA HSTGSSRLHD LYWQAMKTLG VQRPKLEKKD AKEIPSATQS PISKKRKKKG FLPETKKRKK RKSEDGTPAE DGTPAATGGS QPPSMGRKKR NRTKAKVPAQ ANGTPTTKSP APGAPTRSPS TPAKSPKLQK KNQKPSQVNG APGSPTEPAG QKQHQKALPK KGVLGKSPLS ALARKKARLS LVIRSPSLLQ SGAKKKAQVR KAGKP; Positive Samples: HeLa, SW620, MCF7, Mouse liver, Rat liver; Cellular Location: Cytoplasm, Nucleus, nucleolus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.60 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MYBBP1A in extracts of various cell lines using MYBBP1A Polyclonal Antibody (Product # PA5-96287) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of MYBBP1A was performed in U2OS cells using MYBBP1A Polyclonal Antibody (Product # PA5-96287) at a dilution of 1:200. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MYBBP1A in paraffin-embedded human stomach using MYBBP1A Polyclonal Antibody (Product # PA5-96287) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MYBBP1A in paraffin-embedded rat lung. Samples were stained with MYBBP1A polyclonal antibody (Product # PA5-96287) using dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MYBBP1A in paraffin-embedded rat spleen. Samples were stained with MYBBP1A polyclonal antibody (Product # PA5-96287) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
