NB100-61050

MYBBP1A antibody from Novus Biologicals

FLJ37886, P160, PAP2, Pol5

Western blot

Data presented on provider website

Immunoprecipitation

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Proximity ligation assay

Data presented on provider website

Provider product page for NB100-61050

Antibody Data

Product number

NB100-61050

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-61050, RRID:AB_925527

Product name

Rabbit Polyclonal MYBBP1A Antibody

Provider product page

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Comprehensive in vivo identification of the c-Myc mRNA protein interactome using HyPR-MS.
Spiniello M, Steinbrink MI, Cesnik AJ, Miller RM, Scalf M, Shortreed MR, Smith LM
RNA (New York, N.Y.) 2019 Oct;25(10):1337-1352

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TPPII, MYBBP1A and CDK2 form a protein-protein interaction network.
Nahálková J, Tomkinson B
Archives of biochemistry and biophysics 2014 Dec 15;564:128-35

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: MYBBP1A Antibody [NB100-61050] - Detection of Human MYBBP1a by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MYBBP1a antibody NB100-61050 used for IP at 3 mcg/mg lysate. MYBBP1a was also immunoprecipitated by rabbit anti-MYBBP1a antibody NB100-61051, which recognizes a downstream epitope.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: MYBBP1A Antibody [NB100-61050] - Detection of human MYBBP1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MYBBP1A antibody NB100-61050 used for IP at 3 ug per reaction. MYBBP1A was also immunoprecipitated by rabbit anti-MYBBP1A antibody NB100-61051. For blotting immunoprecipitated MYBBP1A, NB100-61051 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MYBBP1A Antibody [NB100-61050] - Human colon carcinoma. Antibody: Affinity purified rabbit anti-MYBBP1A used at a dilution of 1:1,000 (1ug/ml). Detection: DAB