MA5-15515

antibody from Invitrogen Antibodies

Targeting: ISL1 Isl-1, ISLET1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15515

Provider

Invitrogen Antibodies

Product name

ISL1 Monoclonal Antibody (1H9)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15515 targets ISL1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15515 immunogen is purified recombinant fragment of human ISL1 expressed in E. Coli. MA5-15515 detects ISL1 which has a predicted molecular weight of approximately 39kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1H9

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes.

Schörnig M, Ju X, Fast L, Ebert S, Weigert A, Kanton S, Schaffer T, Nadif Kasri N, Treutlein B, Peter BM, Hevers W, Taverna E
eLife 2021 Jan 20;10

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Herpes simplex virus type 1 infection leads to neurodevelopmental disorder-associated neuropathological changes.

Qiao H, Guo M, Shang J, Zhao W, Wang Z, Liu N, Li B, Zhou Y, Wu Y, Chen P
PLoS pathogens 2020 Oct;16(10):e1008899

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Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system.

Wang Y, Wang L, Guo Y, Zhu Y, Qin J
RSC advances 2018 Jan 2;8(3):1677-1685

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ISL1 using ISL1 monoclonal antibody (Product # MA5-15515) in full-length ISL1 (aa1-349) human IgG Fc transfected HEK293 cell lysate (1).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-human IgG Fc using ISL1 monoclonal antibody (Product # MA5-15515) (Green). Blue: DRAQ5 fluorescent DNA dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-human IgG Fc using ISL1 monoclonal antibody (Product # MA5-15515) (Green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and cervical carcinoma (right) using ISL1 monoclonal antibody (Product # MA5-15515) followed with DAB staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 5 Dysregulated the cortical layer and hindbrain populations in HSV-1-infected neuroepithelial buds. (A) The dorsal NSC marker (PAX6) and newborn neuron marker (TUJ) were observed in the brain cerebral organoids at the 18 day after 3 days with HSV-1 infection (Mock, Low infection). Scale bars: 25 mum. The images were taken Images were captured on a Leica TCS SP8 STED confocal microscope. (B) Comparison of depths of CP layer between mock- and HSV-1-infected neuroepithelial buds. (C) Immunofluorescence staining for the hindbrain marker (ISL1) in neuroepithelial buds at the day 18 after 3 days with HSV-1 infection. Scale bars: 25 mum. (D) Quantification of ISL1-positive cells in the presence or absence of HSV-1 infection. Data represent the mean +- SEM from four experiments. **p < 0.01 by Student's t test.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Identification of specific brain regions in brain organoids. (A) Forebrain markers (PAX6, FOXG1) and hindbrain markers (PAX2, ISL1) were quantified by RT-PCR in spheroids at 10 and 16 days of differentiation. The expression values were normalized to the beta-actin expression level. Three independent experiments had been performed. Data are shown as mean +- SD. * p < 0.05, ** p < 0.01. (B) Immunohistochemical staining of PAX6 and PAX2 markers in cryosections of spheroids at 16 days and 33 days. (C) Immunohistochemical staining of PAX6 and ISL1 markers in cryosections of brain organoids at 33 days. Scale bars = 50 mum.