Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AF1837 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Islet-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Islet-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Islet-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P61371
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Gene activation of SMN by selective disruption of lncRNA-mediated recruitment of PRC2 for the treatment of spinal muscular atrophy.
Foxo1 is a downstream effector of Isl1 in direct pathway striatal projection neuron development within the embryonic mouse telencephalon.
Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells.
Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells.
Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells.
LIM-homeodomain transcription factor Isl-1 mediates the effect of leptin on insulin secretion in mice.
Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells.
Inhibitory Smads differentially regulate cell fate specification and axon dynamics in the dorsal spinal cord.
Directed differentiation of human-induced pluripotent stem cells generates active motor neurons.
Woo CJ, Maier VK, Davey R, Brennan J, Li G, Brothers J 2nd, Schwartz B, Gordo S, Kasper A, Okamoto TR, Johansson HE, Mandefro B, Sareen D, Bialek P, Chau BN, Bhat B, Bullough D, Barsoum J
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 21;114(8):E1509-E1518
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 21;114(8):E1509-E1518
Foxo1 is a downstream effector of Isl1 in direct pathway striatal projection neuron development within the embryonic mouse telencephalon.
Waclaw RR, Ehrman LA, Merchan-Sala P, Kohli V, Nardini D, Campbell K
Molecular and cellular neurosciences 2017 Apr;80:44-51
Molecular and cellular neurosciences 2017 Apr;80:44-51
Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells.
Milani P, Escalante-Chong R, Shelley BC, Patel-Murray NL, Xin X, Adam M, Mandefro B, Sareen D, Svendsen CN, Fraenkel E
Scientific reports 2016 May 5;6:25474
Scientific reports 2016 May 5;6:25474
Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
Gu Z, Imai F, Kim IJ, Fujita H, Katayama Ki, Mori K, Yoshihara Y, Yoshida Y
PloS one 2015;10(3):e0121550
PloS one 2015;10(3):e0121550
Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells.
Adams KL, Rousso DL, Umbach JA, Novitch BG
Nature communications 2015 Apr 14;6:6778
Nature communications 2015 Apr 14;6:6778
Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells.
Li R, Wu F, Ruonala R, Sapkota D, Hu Z, Mu X
PloS one 2014;9(3):e92105
PloS one 2014;9(3):e92105
LIM-homeodomain transcription factor Isl-1 mediates the effect of leptin on insulin secretion in mice.
Chen J, Fu R, Cui Y, Li YS, Pan JR, Liu JL, Luo HS, Yin JD, Li DF, Cui S
The Journal of biological chemistry 2013 Apr 26;288(17):12395-405
The Journal of biological chemistry 2013 Apr 26;288(17):12395-405
Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells.
Yau WW, Tang MK, Chen E, Yaoyao, Wong IW, Lee HS, Lee KKh
Proteome science 2011 Jan 19;9(1):3
Proteome science 2011 Jan 19;9(1):3
Inhibitory Smads differentially regulate cell fate specification and axon dynamics in the dorsal spinal cord.
Hazen VM, Phan KD, Hudiburgh S, Butler SJ
Developmental biology 2011 Aug 15;356(2):566-75
Developmental biology 2011 Aug 15;356(2):566-75
Directed differentiation of human-induced pluripotent stem cells generates active motor neurons.
Karumbayaram S, Novitch BG, Patterson M, Umbach JA, Richter L, Lindgren A, Conway AE, Clark AT, Goldman SA, Plath K, Wiedau-Pazos M, Kornblum HI, Lowry WE
Stem cells (Dayton, Ohio) 2009 Apr;27(4):806-11
Stem cells (Dayton, Ohio) 2009 Apr;27(4):806-11
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Islet-1 by Simple WesternTM. Simple Western lane view shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiated into motor neurons, loaded at 0.2 mg/mL. A specific band was detected for Islet-1 at approximately 53 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Islet-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Islet-1 by Western Blot. Western blot shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiatied into motor neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Islet-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Islet-1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Islet-1 in iPS2 Human Stem Cells. Islet-1 was detected in immersion fixed iPS2 human induced pluripotent stem cells differentiated to endocrine progenitor cells using Goat Anti-Human Islet-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.