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antibody from Invitrogen Antibodies
Targeting: KLRK1
CD314, D12S2489E, KLR, NKG2-D, NKG2D
Immunoprecipitation
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 14-5878-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD314 (NKG2D) Monoclonal Antibody (1D11), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells. Applications Reported: The 1D11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in inhibition of ligand binding in in vitro assays. Please use Functional Grade Purified 1D11 (Product # 16-5878) in functional assays. (Fluorochrome-conjugated 1D11 is recommended for use in flow cytometric analysis). Applications Tested: The 1D11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1D11
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references miR-20a regulates sensitivity of colorectal cancer cells to NK cells by targeting MICA.
Functional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production.
Naringenin prevents TGF-β1 secretion from breast cancer and suppresses pulmonary metastasis by inhibiting PKC activation.
Regulatory Dendritic Cells Restrain NK Cell IFN-γ Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
MHC-I molecules selectively inhibit cell-mediated cytotoxicity triggered by ITAM-coupled activating receptors and 2B4.
Chemotherapy sensitizes colon cancer initiating cells to Vγ9Vδ2 T cell-mediated cytotoxicity.
Signaling by Fyn-ADAP via the Carma1-Bcl-10-MAP3K7 signalosome exclusively regulates inflammatory cytokine production in NK cells.
Monocytes and γδ T cells control the acute-phase response to intravenous zoledronate: insights from a phase IV safety trial.
Histone deacetylase inhibitors impair NK cell viability and effector functions through inhibition of activation and receptor expression.
Activating signals dominate inhibitory signals in CD137L/IL-15 activated natural killer cells.
Epitope mapping, expression and post-translational modifications of two isoforms of CD33 (CD33M and CD33m) on lymphoid and myeloid human cells.
The NKG2D ligand ULBP4 binds to TCRgamma9/delta2 and induces cytotoxicity to tumor cells through both TCRgammadelta and NKG2D.
NKG2D-dependent effector function of bronchial epithelium-activated alloreactive T-cells.
Zoonotic orthopoxviruses encode a high-affinity antagonist of NKG2D.
Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis.
NKG2D receptors induced by IL-15 costimulate CD28-negative effector CTL in the tissue microenvironment.
Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA.
Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells.
Tang S, Fu H, Xu Q, Zhou Y
Bioscience reports 2019 Jul 31;39(7)
Bioscience reports 2019 Jul 31;39(7)
Functional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production.
Yin W, Tong S, Zhang Q, Shao J, Liu Q, Peng H, Hu H, Peng M, Hu P, Ren H, Tian Z, Zhang D
Scientific reports 2016 May 19;6:26296
Scientific reports 2016 May 19;6:26296
Naringenin prevents TGF-β1 secretion from breast cancer and suppresses pulmonary metastasis by inhibiting PKC activation.
Zhang F, Dong W, Zeng W, Zhang L, Zhang C, Qiu Y, Wang L, Yin X, Zhang C, Liang W
Breast cancer research : BCR 2016 Apr 1;18(1):38
Breast cancer research : BCR 2016 Apr 1;18(1):38
Regulatory Dendritic Cells Restrain NK Cell IFN-γ Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
Spallanzani RG, Torres NI, Avila DE, Ziblat A, Iraolagoitia XL, Rossi LE, Domaica CI, Fuertes MB, Rabinovich GA, Zwirner NW
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2141-8
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2141-8
MHC-I molecules selectively inhibit cell-mediated cytotoxicity triggered by ITAM-coupled activating receptors and 2B4.
Corral-San Miguel R, Hernández-Caselles T, Ruiz Alcaraz AJ, Martínez-Esparza M, García-Peñarrubia P
PloS one 2014;9(9):e107054
PloS one 2014;9(9):e107054
Chemotherapy sensitizes colon cancer initiating cells to Vγ9Vδ2 T cell-mediated cytotoxicity.
Todaro M, Orlando V, Cicero G, Caccamo N, Meraviglia S, Stassi G, Dieli F
PloS one 2013;8(6):e65145
PloS one 2013;8(6):e65145
Signaling by Fyn-ADAP via the Carma1-Bcl-10-MAP3K7 signalosome exclusively regulates inflammatory cytokine production in NK cells.
Rajasekaran K, Kumar P, Schuldt KM, Peterson EJ, Vanhaesebroeck B, Dixit V, Thakar MS, Malarkannan S
Nature immunology 2013 Nov;14(11):1127-36
Nature immunology 2013 Nov;14(11):1127-36
Monocytes and γδ T cells control the acute-phase response to intravenous zoledronate: insights from a phase IV safety trial.
Welton JL, Morgan MP, Martí S, Stone MD, Moser B, Sewell AK, Turton J, Eberl M
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2013 Mar;28(3):464-71
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2013 Mar;28(3):464-71
Histone deacetylase inhibitors impair NK cell viability and effector functions through inhibition of activation and receptor expression.
Rossi LE, Avila DE, Spallanzani RG, Ziblat A, Fuertes MB, Lapyckyj L, Croci DO, Rabinovich GA, Domaica CI, Zwirner NW
Journal of leukocyte biology 2012 Feb;91(2):321-31
Journal of leukocyte biology 2012 Feb;91(2):321-31
Activating signals dominate inhibitory signals in CD137L/IL-15 activated natural killer cells.
Zhang H, Cui Y, Voong N, Sabatino M, Stroncek DF, Morisot S, Civin CI, Wayne AS, Levine BL, Mackall CL
Journal of immunotherapy (Hagerstown, Md. : 1997) 2011 Mar;34(2):187-95
Journal of immunotherapy (Hagerstown, Md. : 1997) 2011 Mar;34(2):187-95
Epitope mapping, expression and post-translational modifications of two isoforms of CD33 (CD33M and CD33m) on lymphoid and myeloid human cells.
Pérez-Oliva AB, Martínez-Esparza M, Vicente-Fernández JJ, Corral-San Miguel R, García-Peñarrubia P, Hernández-Caselles T
Glycobiology 2011 Jun;21(6):757-70
Glycobiology 2011 Jun;21(6):757-70
The NKG2D ligand ULBP4 binds to TCRgamma9/delta2 and induces cytotoxicity to tumor cells through both TCRgammadelta and NKG2D.
Kong Y, Cao W, Xi X, Ma C, Cui L, He W
Blood 2009 Jul 9;114(2):310-7
Blood 2009 Jul 9;114(2):310-7
NKG2D-dependent effector function of bronchial epithelium-activated alloreactive T-cells.
Kraetzel K, Stoelcker B, Eissner G, Multhoff G, Pfeifer M, Holler E, Schulz C
The European respiratory journal 2008 Sep;32(3):563-70
The European respiratory journal 2008 Sep;32(3):563-70
Zoonotic orthopoxviruses encode a high-affinity antagonist of NKG2D.
Campbell JA, Trossman DS, Yokoyama WM, Carayannopoulos LN
The Journal of experimental medicine 2007 Jun 11;204(6):1311-7
The Journal of experimental medicine 2007 Jun 11;204(6):1311-7
Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis.
Groh V, Bruhl A, El-Gabalawy H, Nelson JL, Spies T
Proceedings of the National Academy of Sciences of the United States of America 2003 Aug 5;100(16):9452-7
Proceedings of the National Academy of Sciences of the United States of America 2003 Aug 5;100(16):9452-7
NKG2D receptors induced by IL-15 costimulate CD28-negative effector CTL in the tissue microenvironment.
Roberts AI, Lee L, Schwarz E, Groh V, Spies T, Ebert EC, Jabri B
Journal of immunology (Baltimore, Md. : 1950) 2001 Nov 15;167(10):5527-30
Journal of immunology (Baltimore, Md. : 1950) 2001 Nov 15;167(10):5527-30
Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA.
Bauer S, Groh V, Wu J, Steinle A, Phillips JH, Lanier LL, Spies T
Science (New York, N.Y.) 1999 Jul 30;285(5428):727-9
Science (New York, N.Y.) 1999 Jul 30;285(5428):727-9
Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells.
Groh V, Steinle A, Bauer S, Spies T
Science (New York, N.Y.) 1998 Mar 13;279(5357):1737-40
Science (New York, N.Y.) 1998 Mar 13;279(5357):1737-40
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.06 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.06 µg of Anti-Human CD314 (NKG2D) Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85).Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 miR-20a regulates CRC cells sensitivity to NK cells depending on NKG2D signaling ( A ) Depletion of NK cells markedly abolished the cell cytotoxicity. CD56 + NK cells were depleted from PBMCs by magnetic sorting. NK cell-depleted PBMCs were then co-cultured with miR-20a overexpressing and knockdown of CRC cells at an E:T ratio of 50:1 for 16 h. ( B ) NK cells were co-cultured with miR-20a overexpressing and knockdown CRC cells at an E:T ratio of 50:1 in the presence or absence of NKG2D blocking antibody for 16 h. The specific kill of target cells were examined by FACS. All data were shown as mean +- S.E.M. from three independent experiments. * represents P
