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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 14277-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COG6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: VGDMLAWLH QATASEKEHL EALLKHVTTQ GVEENIQEVV GHITEGVCRP LKVRIEQVIV AEPGAVLLYK ISNLLKFYHH TISGIVGNSA TALLTTIEEM HLLSKKIFFN SLSLHASKLM DKVELPPPDL GPSSALNQTL MLLREVLASH DSSVVPLDAR QADFVQVLSC VLDPLLQMCT VSASNLGTAD MATFMVNSLY MMKTTLALFE FTDRRLEMLQ FQIEAHLDTL INEQASYVLT RVGLSYIYNT VQQHKPEQGS LANMPNLDSV TLKAAMVQFD RYLSAPDNLL IPQLNFLLSA TVKEQIVKQS TELVCRAYGE VYAAVMNPIN EYKDPENILH RSPQQVQTLL S (308-657 aa encoded by BC051723)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.56 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using COG6 antibody 14277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using COG6 antibody 14277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 MCF-7 cells were stained with 0.2ug COG6 antibody (14277-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
