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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- V3371 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- FOXA1 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific FOXA1 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Immunofluorescence applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- FOXA1/1518
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the FOXA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HeLa cell lysate with FOXA1 antibody (clone FOXA1/1518). Predicted molecular weight: ~49 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PFA-fixed human MCF7 cells with FOXA1 antibody (clone FOXA1/1518, green) and Phalloiden (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1518). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat colon with FOXA1 antibody (clone FOXA1/1518). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat colon with FOXA1 antibody (clone FOXA1/1518). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of PFA-fixed human MCF-7 cells with FOXA1 antibody (clone FOXA1/1518); Red=isotype control, Blue= FOXA1 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free FOXA1 Antibody (clone FOXA1/1518). Confirmation of Integrity and Purity of the Antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free FOXA1 Antibody (clone FOXA1/1518). Confirmation of Integrity and Purity of the Antibody.
