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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00028227-M08 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00028227-M08, RRID:AB_1136994
- Product name
- PPP2R3B monoclonal antibody (M08), clone 2E12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant PPP2R3B.
- Antigen sequence
MIDRIFSGAVTRGRKVQKEGKISYADFVWFLISEE
DKKTPTSIEYWFRCMDLDGDGALSMFELEYFYEEQ
CRRLDSMAIEALPFQDCLCQMLDLVKPRTEGKITL
QDLKRCKLANVFFDTFFNIEKYLDHEQKEQISLLR
DGDSGGPELSDWEKYAAEEYDILVAEETAGEPWED
GFEAELSPVEQKLSALRSPLAQRPFFEAPSPLGAV
DLYEYACGDEDLEPL- Isotype
- IgG
- Antibody clone number
- 2E12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PPP2R3B is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PPP2R3B on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PPP2R3B transfected lysate using anti-PPP2R3B monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PPP2R3B MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
