10582-1-AP

antibody from Proteintech Group

Targeting: PELO

Provider product page for 10582-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [18]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 10582-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10582-1-AP, RRID:AB_2236833
Product name: PELO antibody
Antibody type: Polyclonal
Description: PELO antibody (Cat. #10582-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PELO protein structure - 10582-1-AP shown in red.

Submitted references The coordinated action of UFMylation and the RQC pathways clears arrested polypeptides at the ER.

Mihailovic M, Anisimova AS, Erte B, Zhan N, Styliara I, Dagdas Y, Karagöz GE
The EMBO journal 2026 May;45(9):3252-3275

Global genetic interaction network of a human cell maps conserved principles and informs functional interpretation of gene co-essentiality profiles.

Billmann M, Costanzo M, Zhang X, Hassan AZ, Rahman M, Brown KR, Chan KS, Tong AHY, Pons C, Ward HN, Ross C, van Leeuwen J, Aregger M, Lawson KA, Mair B, Roth AF, Sen NE, Forster DT, Tan G, Mero P, Masud SN, Lee Y, Aguilera-Uribe M, Ušaj M, Almeida SMT, Aulakh K, Bhojoo U, Birkadze S, Budijono N, Cai X, Caumanns JJ, Chalmers JJ, Chandrashekhar M, Chang D, Climie R, Dasgupta K, Drazic A, Echenique JIR, Gacesa R, Farias AG, Habsid A, Horecka I, Kantautas K, Ji F, Kim DK, Lee SY, Liang W, Lim HJ, Lin K, Lu X, Maier M, Nami B, Nixon A, Mikolajewicz N, Mokhtaridoost M, Nedyalkova L, Rohde T, Rodrigues MS, Soste M, Schultz E, Wang W, Seetharaman A, Shuteriqi E, Sizova O, Taylor DT, Tereshchenko M, Tieu D, Turowec J, Ubhi T, Varland S, Wang KE, Wang ZY, Wei J, Xiao YX, Maass PG, Reversade B, Brown GW, Cravatt BF, Dixon SJ, Wyatt HDM, Röst HL, Roth FP, Xia T, Bader GD, Loewith R, Davis NG, Andrews B, Myers CL, Moffat J, Boone C
Cell 2026 Apr 27;

Translation regulation of ATF4 by the termination complex Hbs1-Pelo is required for visual system development and function.

Querry K, Lago-Baldaia I, Nampoothiri V P N, Garbark C, Carney AJ, Fernandes VM, Vasudevan D
bioRxiv : the preprint server for biology 2025 Oct 8;

Molecular subtyping of endometrial carcinoma cell lines uncovers subtype-specific targetable vulnerabilities.

Li ES, Ho R, Tao R, Choi YWY, Shin CY, Chen SY, Gokbayrak B, Senz J, Yao B, Johnston L, Martin SD, Yang E, Carey MS, Hennessy BT, Huntsman DG, Klein Geltink RI, Hoang L, Wang Y
NPJ precision oncology 2025 Jul 24;9(1):254

Synthetic lethality of mRNA quality control complexes in cancer.

Prindle V, Richardson AE, Sher KR, Kongpachith S, Kentala K, Petiwala S, Cheng D, Widomski D, Le P, Torrent M, Chen A, Walker S, Palczewski MB, Mitra D, Manaves V, Shi X, Lu C, Sandoval S, Dezso Z, Buchanan FG, Verduzco D, Bierie B, Meulbroek JA, Pappano WN, Plotnik JP
Nature 2025 Feb;638(8052):1095-1103

Translation stalling induced mitochondrial entrapment of ribosomal quality control related proteins offers cancer cell vulnerability.

Ojha R, Tantray I, Banerjee S, Rimal S, Thirunavukkarasu S, Srikrishna S, Chiu W, Mete U, Sharma A, Kakkar N, Lu B
Research square 2024 Sep 13;

PELO regulates erythroid differentiation through interaction with MYC to upregulate KLF10.

Hao J, Han G, Liang X, Ruan Y, Huang C, Sa N, Hu H, Hu B, Li Z, Zhang K, Gao P, Dong X
The FEBS journal 2024 Nov;291(21):4714-4731

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis.

Geng J, Li S, Li Y, Wu Z, Bhurtel S, Rimal S, Khan D, Ohja R, Brandman O, Lu B
Nature communications 2024 Feb 22;15(1):1637

HBS1L deficiency causes retinal dystrophy in a child and in a mouse model associated with defective development of photoreceptor cells.

Luo S, Alwattar B, Li Q, Bora K, Blomfield AK, Lin J, Fulton A, Chen J, Agrawal PB
Disease models & mechanisms 2024 Aug 1;17(8)

Single-molecule imaging reveals distinct elongation and frameshifting dynamics between frames of expanded RNA repeats in C9ORF72-ALS/FTD.

Latallo MJ, Wang S, Dong D, Nelson B, Livingston NM, Wu R, Zhao N, Stasevich TJ, Bassik MC, Sun S, Wu B
Nature communications 2023 Sep 11;14(1):5581

Genetic deficiency of ribosomal rescue factor HBS1L causes retinal dystrophy associated with Pelota and EDF1 depletion.

Luo S, Alwattar B, Li Q, Bora K, Blomfield AK, Lin J, Fulton A, Chen J, Agrawal PB
bioRxiv : the preprint server for biology 2023 Oct 20;

Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity.

Wu X, Yang ZH, Wu J, Han J
Immunity 2023 May 9;56(5):926-943.e7

Protocol for reconstitution of oligomeric assembly of NAIP5-NLRC4 inflammasome in vitro.

Wu X, Han J
STAR protocols 2023 Dec 15;4(4):102581

Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress.

Stoneley M, Harvey RF, Mulroney TE, Mordue R, Jukes-Jones R, Cain K, Lilley KS, Sawarkar R, Willis AE
Molecular cell 2022 Apr 21;82(8):1557-1572.e7

A role for the ribosome-associated complex in activation of the IRE1 branch of UPR.

Wu IH, Yoon JS, Yang Q, Liu Y, Skach W, Thomas P
Cell reports 2021 Jun 8;35(10):109217

Defects in translation-dependent quality control pathways lead to convergent molecular and neurodevelopmental pathology.

Terrey M, Adamson SI, Chuang JH, Ackerman SL
eLife 2021 Apr 26;10

XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.

Hou S, Qu D, Li Y, Zhu B, Liang D, Wei X, Tang W, Zhang Q, Hao J, Guo W, Wang W, Zhao S, Wang Q, Azam S, Khan M, Zhao H, Zhang L, Lei H
Nucleic acids research 2019 Sep 5;47(15):8239-8254

Mammalian Hbs1L deficiency causes congenital anomalies and developmental delay associated with Pelota depletion and 80S monosome accumulation.

O'Connell AE, Gerashchenko MV, O'Donohue MF, Rosen SM, Huntzinger E, Gleeson D, Galli A, Ryder E, Cao S, Murphy Q, Kazerounian S, Morton SU, Schmitz-Abe K, Gladyshev VN, Gleizes PE, Séraphin B, Agrawal PB
PLoS genetics 2019 Feb;15(2):e1007917

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10582-1-AP(PELO antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human ovary tumor using 10582-1-AP(PELO antibody) at dilution of 1:100 (under 25x lens)
Sample type: tissue