PA5-31697

antibody from Invitrogen Antibodies

Targeting: PELO

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31697

Provider

Invitrogen Antibodies

Product name

PELO Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, HeLa, HepG2, PC-12, Rat2, , Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12. Predicted reactivity: Human (99%), Mouse (97%), Rat (97%), Zebrafish (82%), Xenopus laevis (83%), Chicken (95%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Coupled protein synthesis and ribosome-guided piRNA processing on mRNAs.

Sun YH, Wang RH, Du K, Zhu J, Zheng J, Xie LH, Pereira AA, Zhang C, Ricci EP, Li XZ
Nature communications 2021 Oct 13;12(1):5970

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PELO showing staining in the cytoplasm and nucleus of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a PELO polyclonal antibody (Product # PA5-31697) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PELO Polyclonal Antibody detects PELO protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PELO protein stained by PELO Polyclonal Antibody (Product # PA5-31697) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using PELO (Product # PA5-31697) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 3'UTR piRNA biogenesis as a special co-translational mRNA decay pathway. a Immunolabeling of squashed pachytene spermatocytes (left) and round spermatids (right). Three examples of each stage were shown using anti-PELOTA, DAPI, and merged. Experiments were repeated at least three times independently with similar results. Scale bar, 5 mum. b Boxplots of the ratios of RNA abundance in UPF1 IP (upper) and phosphorylated UPF1 IP mutant (lower) versus their corresponding input in adult wild-type testes. Control mRNA n = 43, uppl mRNA n = 30. c Boxplots of the changes of protein abundance coded per gene in Mov10l1 mutants (sample size n = 5 independent biological replicates) compared to littermate controls in adult testes. Control mRNA n = 43, uppl mRNA n = 30. d AGO2 western blot (top) and TUBULIN western blot (bottom) of adult testis lysates from serial dilutions of adult wild-type lysates (left) and four biological replicates of Mov10l1 CKO/ Neurog3-cre (middle) and Mov10l1 CKO/ (right). Abundance of AGO2 and TUBULIN was quantified using ImageJ and then fitted to the standard curve of the wild-type testis lysates. Relative abundance of AGO2 were calculated as the abundance of AGO2 normalized by the abundance of TUBULIN (sample size n = 4 independent biological samples). Data are mean +- standard deviation; * p = 0.048, one-side Student's t test. Each data point was overlaid as dot plots. e Boxplots of the 5'P decay intermediates per mRNA in adult wild-type testes. AGO2 ta