NBP2-76406

antibody from Novus Biologicals

Targeting: VSIR B7-H5, B7H5, C10orf54, Dies1, GI24, PD-1H, SISP1, VISTA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-76406

Provider

Novus Biologicals

Product name

Rabbit Monoclonal VISTA/B7-H5/PD-1H Antibody

Antibody type

Monoclonal

Description

Antigen Affinity-purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at 2 - 8 C 1 year from date of receipt

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA by WB of Jurkat, MOLT-4, MJ, RPMI-8226, HeLa, KG-1, HEK293T, and SUP-M2 lysate. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406). Secondary: HRP-conjugated goat anti-rabbit IgG.Lower Panel: Rabbit anti-GAPDH.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA by WB of immunoprecipitates from Jurkat lysate. Antibodies: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406) and another rabbit anti-VISTA recombinant monoclonal.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.