V7537

antibody from NSJ Bioreagents

Targeting: VSIR B7-H5, B7H5, C10orf54, Dies1, GI24, PD-1H, SISP1, VISTA

Immunohistochemistry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V7537

Provider

NSJ Bioreagents

Product name

Vista Antibody

Antibody type

Monoclonal

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placenta with VISTA antibody (clone VISTA/2864). HIER: boil tissue sections in pH 9, 10mM Tris with 1mM EDTA, for 10-20 min and allow to cool before testing.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using VISTA antibody (clone VISTA/2864). These results demonstrate the foremost specificity of the VISTA/2864 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free VISTA antibody (clone VISTA/2864) as confirmation of integrity and purity.