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LearnA700-035-T
antibody from Invitrogen Antibodies
Targeting: VSIR
B7-H5, B7H5, C10orf54, Dies1, GI24, PD-1H, SISP1, VISTA
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- A700-035-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VISTA Recombinant Rabbit Monoclonal Antibody (BLR035F)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR035F
- Vial size
- 10 µL
- Concentration
- 100 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human VISTA by western blot. Samples: Whole cell lysate (50 µg) from HeLa, Jurkat, HEK293T, KG-1, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (Product # A700-035 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (Product # A700-057).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human VISTA by immunocytochemistry.IHC. AntibodySample: FFPE section of NCI-H226 cells.Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (BLR035F) (A700-035 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human VISTA (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (Product # A700-035 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human VISTA in human peripheral blood mononuclear cells by flow cytometry. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (Product #A700-035; lot 1) (right) or isotype control (left). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human VISTA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (Product # A700-035 lot 2) used for IP at 10 µL/mg lysate. VISTA was also immunoprecipitated by a second antibody against a different epitiope of VISTA (BL-435-5G11). For blotting immunoprecipitated VISTA (Product # A700-035) was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
