Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-47646 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LTK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-human LTK Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47646) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for LTK at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LTK in Jurkat human acute T cell leukemia cell line. Samples were incubated in LTK polyclonal antibody (Product # PA5-47646) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for LTK at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LTK in 0.2 mg/mL lysates of Jurkat human acute T cell leukemia cell line. Samples were incubated in LTK polyclonal antibody (Product # PA5-47646) using a dilution of 50 µg/mL followed by HRP-conjugated Anti-Sheep IgG at a dilution of 0.0763888888888889. A specific band was detected for LTK at approximately 77 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LTK in immersion fixed paraffin-embedded sections of human brain (cortex). Samples were incubated in LTK polyclonal antibody (Product # PA5-47646) using a dilution of 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hemotoxylin (blue). Specific staining was localized to neurons.