PA5-88348

antibody from Invitrogen Antibodies

Targeting: SEC23A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88348

Provider

Invitrogen Antibodies

Product name

SEC23A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MTTYLEFIQQ NEERDGVRFS WNVWPSSRLE ATRMVVPVAA LFTPLKERPD LPPIQYEPVL CSRTTCRAVL NPLCQVDYRA KLWACNFCYQ RNQFPPSYAG ISELNQPAEL LPQFSSIEYV VLRGPQMPLI FLYVVDTCME DEDLQALKES; Positive Samples: B-cell, LO2, HeLa, SGC-7901, HT-29, Mouse brain, Mouse liver, Rat brain; Cellular Location: Golgi apparatus membrane, Peripheral membrane protein, Smooth endoplasmic reticulum membrane

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.85 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using SEC23A Polyclonal antibody (Product # PA5-88348) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SEC23A in extracts of various cell lines using SEC23A Polyclonal Antibody (Product # PA5-88348) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.