Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-28984 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEC23A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 1 mg/mL
Submitted references ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.
Taftaf R, Liu X, Singh S, Jia Y, Dashzeveg NK, Hoffmann AD, El-Shennawy L, Ramos EK, Adorno-Cruz V, Schuster EJ, Scholten D, Patel D, Zhang Y, Davis AA, Reduzzi C, Cao Y, D'Amico P, Shen Y, Cristofanilli M, Muller WA, Varadan V, Liu H
Nature communications 2021 Aug 11;12(1):4867
Nature communications 2021 Aug 11;12(1):4867
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SEC23A Polyclonal Antibody (Product # PA5-28984). Sample (30 µg of whole cell lysate). A: Molt-4. 7.5% SDS PAGE. SEC23A Polyclonal Antibody (Product # PA5-28984) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SEC23A was achieved by transfecting A549 cells with SEC23A specific siRNAs (Silencer® select Product # s20538, s20539). Western blot analysis (Fig. a) was performed using whole cell extracts from the SEC23A knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SEC23A Polyclonal Antibody (Product # PA5-28984, 1:10000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SEC23A.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extract (30 µg lysate) of U-87MG (Lane 1), A549 (Lane 2), THP-1 (Lane 3), MOLT-4 (Lane 4), T-47D (Lane 5), HepG2 (Lane 6), HeLa (Lane 7), C2C12 (Lane 8), L6 (Lane 9) and HCT116 (Lane 10). The blot was probed with Anti-SEC23A Polyclonal Antibody (Product # PA5-28984, 1:10000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 86 kDa band corresponding to SEC23A was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SEC23A in methanol-fixed HeLa cells using a SEC23A polyclonal antibody (Product # PA5-28984) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SEC23A was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SEC23A Polyclonal Antibody (Product # PA5-28984) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SEC23A (Product # PA5-28984) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SEC23A Polyclonal Antibody detects SEC23A protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. SEC23A Polyclonal Antibody (Product # PA5-28984) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.