NBP1-85146

antibody from Novus Biologicals

Targeting: ACBD5 DKFZp434A2417, KIAA1996

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85146

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85146, RRID:AB_11006768

Product name

Rabbit Polyclonal ACBD5 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human ACBD5 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Peroxisomal Atg37 binds Atg30 or palmitoyl-CoA to regulate phagophore formation during pexophagy.

Nazarko TY, Ozeki K, Till A, Ramakrishnan G, Lotfi P, Yan M, Subramani S
The Journal of cell biology 2014 Feb 17;204(4):541-57

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ACBD5 Antibody [NBP1-85146] - Analysis in human cell lines A-549 and PC-3 using anti-ACBD5 antibody. Corresponding ACBD5 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining of human pancreas shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining of human parathyroid gland shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining in human parathyroid gland and pancreas tissues using anti-ACBD5 antibody. Corresponding ACBD5 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining of human adrenal gland.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining of human testis.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining of human liver.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining of human colon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining of human pancreas shows very weak granular cytoplasmic positivity in exocrine glandular cells

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Analysis in human small intestine and pancreas tissues using antibody. Corresponding ACBD5 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining of human kidney, liver, pancreas and small intestine using Anti-ACBD5 antibody NBP1-85146 (A) shows similar protein distribution across tissues to independent antibody NBP1-85145 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACBD5 Antibody [NBP1-85146] - Staining of human kidney, liver, pancreas and small intestine using Anti-ACBD5 antibody NBP1-85146 (A) shows similar protein distribution across tissues to independent antibody NBP1-85145 (B).