Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [2]
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- Product number
- 12-5499-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSLP Receptor Monoclonal Antibody (eBio1A6 (1A6)), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBio1A6 monoclonal antibody reacts with human thymic stromal-derived lymphopoietin receptor (TSLPR). TSLPR is an approximately 50 kDa protein with significant similarity to the common gamma-chain. TSLPR complexes with IL-7R alpha (CD127) to form the high affinity receptor that binds thymic stromal-derived lymphopoietin (TSLP). Human TSLPR is expressed by monocytes and CD11c+ dendritic cells, and TSLP binding induces the expression of the Th2 cell-attracting chemokines CCL17 and CCL22. Furthermore, the TSLPR-induced activation of dendritic cells indirectly results in the increased secretion of Th cytokines IL-4, -5 and -13, which may be necessary for the regulation of CD4+ T cell homeostasis. In mice, deficiency of TSLPR has no effect on lymphocyte numbers, whereas double deficiency of TSLPR and common gamma-chain results in fewer lymphocytes than seen in mice deficient in the common gamma-chain alone. The eBio1A6 monoclonal antibody is able to cross-block binding of another anti-human TSLPR monoclonal antibody, 1D8. Applications Reported: This eBio1A6 (1A6) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio1A6 (1A6) antibody has been pre-titrated and tested by flow cytometric analysis of human TSLPR-transfected Ba/F3 cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- eBio1A6 (1A6)
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references JAK2 is dispensable for maintenance of JAK2 mutant B-cell acute lymphoblastic leukemias.
Activity of the Type II JAK2 Inhibitor CHZ868 in B Cell Acute Lymphoblastic Leukemia.
Respiratory syncytial virus induces functional thymic stromal lymphopoietin receptor in airway epithelial cells.
BET bromodomain inhibition targets both c-Myc and IL7R in high-risk acute lymphoblastic leukemia.
Activating mutation in the TSLPR gene in B-cell precursor lymphoblastic leukemia.
Kim SK, Knight DA, Jones LR, Vervoort S, Ng AP, Seymour JF, Bradner JE, Waibel M, Kats L, Johnstone RW
Genes & development 2018 Jun 1;32(11-12):849-864
Genes & development 2018 Jun 1;32(11-12):849-864
Activity of the Type II JAK2 Inhibitor CHZ868 in B Cell Acute Lymphoblastic Leukemia.
Wu SC, Li LS, Kopp N, Montero J, Chapuy B, Yoda A, Christie AL, Liu H, Christodoulou A, van Bodegom D, van der Zwet J, Layer JV, Tivey T, Lane AA, Ryan JA, Ng SY, DeAngelo DJ, Stone RM, Steensma D, Wadleigh M, Harris M, Mandon E, Ebel N, Andraos R, Romanet V, Dölemeyer A, Sterker D, Zender M, Rodig SJ, Murakami M, Hofmann F, Kuo F, Eck MJ, Silverman LB, Sallan SE, Letai A, Baffert F, Vangrevelinghe E, Radimerski T, Gaul C, Weinstock DM
Cancer cell 2015 Jul 13;28(1):29-41
Cancer cell 2015 Jul 13;28(1):29-41
Respiratory syncytial virus induces functional thymic stromal lymphopoietin receptor in airway epithelial cells.
Miazgowicz MM, Elliott MS, Debley JS, Ziegler SF
Journal of inflammation research 2013;6:53-61
Journal of inflammation research 2013;6:53-61
BET bromodomain inhibition targets both c-Myc and IL7R in high-risk acute lymphoblastic leukemia.
Ott CJ, Kopp N, Bird L, Paranal RM, Qi J, Bowman T, Rodig SJ, Kung AL, Bradner JE, Weinstock DM
Blood 2012 Oct 4;120(14):2843-52
Blood 2012 Oct 4;120(14):2843-52
Activating mutation in the TSLPR gene in B-cell precursor lymphoblastic leukemia.
Chapiro E, Russell L, Lainey E, Kaltenbach S, Ragu C, Della-Valle V, Hanssens K, Macintyre EA, Radford-Weiss I, Delabesse E, Cavé H, Mercher T, Harrison CJ, Nguyen-Khac F, Dubreuil P, Bernard OA
Leukemia 2010 Mar;24(3):642-5
Leukemia 2010 Mar;24(3):642-5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of human TSLP Receptor transfected cells with Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (blue histogram) or Anti-Human TSLP Receptor PE (purple histogram). Total viable cells were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye