14-5499-82

antibody from Invitrogen Antibodies
Targeting: CRLF2 CRL2, TSLPR
Provider product page for 14-5499-82

Antibody data

Product number
14-5499-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
TSLP Receptor Monoclonal Antibody (eBio1A6 (1A6)), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The eBio1A6 monoclonal antibody reacts with human thymic stromal-derived lymphopoietin receptor (TSLPR). TSLPR is an approximately 50 kDa protein with significant similarity to the common gamma-chain. TSLPR complexes with IL-7R alpha (CD127) to form the high affinity receptor that binds thymic stromal-derived lymphopoietin (TSLP). Human TSLPR is expressed by monocytes and CD11c+ dendritic cells, and TSLP binding induces the expression of the Th2 cell-attracting chemokines CCL17 and CCL22. Furthermore, the TSLPR-induced activation of dendritic cells indirectly results in the increased secretion of Th cytokines IL-4, -5 and -13, which may be necessary for the regulation of CD4+ T cell homeostasis. In mice, deficiency of TSLPR has no effect on lymphocyte numbers, whereas double deficiency of TSLPR and common gamma-chain results in fewer lymphocytes than seen in mice deficient in the common gamma-chain alone. The eBio1A6 monoclonal antibody is able to cross-block binding of another anti-human TSLPR monoclonal antibody, 1D8. Applications Reported: This eBio1A6 (1A6) antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB). Applications Tested: This eBio1A6 (1A6) antibody has been tested by flow cytometric analysis of human TSLPR-transfected Ba/F3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
eBio1A6 (1A6)
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
CRLF2 protein structure - 14-5499-82 shown in red.