PA5-72543

antibody from Invitrogen Antibodies

Targeting: KLRC1 CD159a, NKG2, NKG2-A, NKG2-B

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-72543

Provider

Invitrogen Antibodies

Product name

NKG2A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

200 μL

Concentration

0.4 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KLRC1 in paraffin-embedded HeLa cells. Sample was 4% paraformaldehyde-fixed, permeabilized with 0.1% Triton X-100 and incubated with KLRC1 polyclonal antibody (Product # PA5-72543) using a 1:25 dilution. Dylight 488-conjugated goat anti-rabbit IgG was used as secondary antibody at a dilution of 1:200 dilution. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1:100 dilution (red) and the nuclear counter stain is DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of NKG2A in Hela (Human Cervical epithelial adenocarcinoma cell line) cells. Samples were incubated with NKG2A polyclonal antibody (Product # PA5-72543) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized. Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NKG2A in formalin-fixed and paraffin-embedded human kidney carcinoma. Samples were incubated with NKG2A polyclonal antibody (Product # PA5-72543) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of KLRC1 in Jurkat cells (green line). The cells were fixed with 2% paraformaldhyde (10 min) and permeabilized with 90% methanol (10 min). The sample was then incubated with 2% BSA followed by a KLRC1 polyclonal antibody (Product # PA5-72543) using a dilution of 1:25 for 60 min at 37°C. The secondary antibody used was goat-anti-rabbit IgG, DyLight 488 with a dilution of 1:200 for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 µg/1x10^6 cells) used under the same conditions with an acquisition of >10, 000 events.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of (overlay histogram) of NKG2A in Jurkat cells (green line). Samples were incubated with NKG2A polyclonal antibody (Product # PA5-72543) using a dilution of 1:25 dilution for 60 min at 37°C followed by Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:400 dilution for 40 min at 37°C. The cells were fixed with 2% paraformaldehyde (10 min). The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the primary antibody. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.