Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-72543 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NKG2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.45 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KLRC1 in Jurkat cell lysate using 35 µg of protein per lane. Samples were incubated with KLRC1 polyclonal antibody (Product # PA5-72543).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KLRC1 in paraffin-embedded HeLa cells. Sample was 4% paraformaldehyde-fixed, permeabilized with 0.1% Triton X-100 and incubated with KLRC1 polyclonal antibody (Product # PA5-72543) using a 1:25 dilution. Dylight 488-conjugated goat anti-rabbit IgG was used as secondary antibody at a dilution of 1:200 dilution. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1:100 dilution (red) and the nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of KLRC1 in paraffin-embedded human kidney carcinoma tissue. Samples were incubated with KLRC1 polyclonal antibody (Product # PA5-72543) followed by a peroxidase-conjugate secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of KLRC1 in Jurkat cells (green line). The cells were fixed with 2% paraformaldhyde (10 min) and permeabilized with 90% methanol (10 min). The sample was then incubated with 2% BSA followed by a KLRC1 polyclonal antibody (Product # PA5-72543) using a dilution of 1:25 for 60 min at 37°C. The secondary antibody used was goat-anti-rabbit IgG, DyLight 488 with a dilution of 1:200 for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 µg/1x10^6 cells) used under the same conditions with an acquisition of >10, 000 events.