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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- PA5-78285 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNAP29 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, A431, HeLa, HepG2, mouse brain, rat brain Predicted Reactivity: Rhesus Monkey (100%), Bovine (84%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.63 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNAP29 in whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with SNAP29 polyclonal antibody (Product # PA5-78285) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNAP29 in various tissue extracts using 50 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with SNAP29 polyclonal antibody (Product # PA5-78285) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SNAP29 Polyclonal Antibody (Product # PA5-78285). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP29 Polyclonal Antibody (Product # PA5-78285) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SNAP29 Polyclonal Antibody (Product # PA5-78285). Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP29 Polyclonal Antibody (Product # PA5-78285) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNAP29 Polyclonal Antibody detects SNAP29 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAP29 protein stained by SNAP29 Polyclonal Antibody (Product # PA5-78285) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
