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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-87632 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ART1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QSHPITRRDL FSQEIQLDMA LASFDDQYAG CAAAMTAALP DLNHTEFQAN QVYADSWTLA SSQWQERQAR WPEWSLSPTR PSPPPLGFRD EHGVALLAYT ANSPLHKEFN AAVREAGRSR AHYLHHFSFK TLHFLLTEAL QLLGSGQRPP RCHQVFRGVH GLRFRPAGPR ATVRLGGFAS ASLKHVAAQQ FGEDTFFGIW TCLGAPIKGY SFFPGEEEVL IPPFETFQVI NASRLAQGPA RIYLRALGKH STYNCEYIKD KKCKSGPCHL DNS; Positive Samples: MCF7, B cells, HepG2; Cellular Location: GPI-anchor, Lipid-anchor, Sarcoplasmic reticulum membrane
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.04 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using ART1 Polyclonal antibody (Product # PA5-87632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ART1 was performed in L929 cells using ART1 Polyclonal Antibody (Product # PA5-87632) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ART1 was performed in U-2 OS cells using ART1 Polyclonal Antibody (Product # PA5-87632).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ART1 in paraffin-embedded human lung cancer using ART1 Polyclonal Antibody (Product # PA5-87632) at a dilution of 1:200.
