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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-31460 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNX18 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, C8D30, PC-12. Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Integrative proteomics reveals an increase in non-degradative ubiquitylation in activated CD4(+) T cells.
Dybas JM, O'Leary CE, Ding H, Spruce LA, Seeholzer SH, Oliver PM
Nature immunology 2019 Jun;20(6):747-755
Nature immunology 2019 Jun;20(6):747-755
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SNX18 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SNX18 Polyclonal Antibody (Product # PA5-31460) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SNX18 Polyclonal Antibody (Product # PA5-31460). Sample (30 µg of whole cell lysate). Lane A: A549 . 7.5% SDS PAGE. SNX18 Polyclonal Antibody (Product # PA5-31460) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SNX18 Polyclonal Antibody (Product # PA5-31460). Sample (30 µg of whole cell lysate). Lane A: C8D30 . 7.5% SDS PAGE. SNX18 Polyclonal Antibody (Product # PA5-31460) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SNX18 in methanol-fixed HeLa cells using a SNX18 polyclonal antibody (Product # PA5-31460) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cal27 xenograft, using SNX18 (Product # PA5-31460) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
