LS-C674962

antibody from LSBio

Targeting: NUP98 NUP96, Nup98-96, Nup98-Nup96

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C674962

Provider

LSBio

Product name

NUP98 Antibody LS-C674962

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Positive Western Blot detected in MCF-7 whole cell lysate, Hela whole cell lysate. All lanes: NUP98 antibody at 3.2 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 198, 188, 98, 97, 196, 187 KDa. Observed band size: 105 KDa

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Positive Western Blot detected in MCF-7 whole cell lysate, Hela whole cell lysate. All lanes: NUP98 antibody at 3.2 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 198, 188, 98, 97, 196, 187 KDa. Observed band size: 105 KDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating NUP98 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of NUP98 Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: NUP98 Antibody (8µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating NUP98 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of NUP98 Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: NUP98 Antibody (8µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating NUP98 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of NUP98 Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: NUP98 Antibody (8µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (20µg)