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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- PA5-144272 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NUP98 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 3.205 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NUP98 in MCF-7 whole cell lysate, HeLa whole cell lysate using a dilution of 3.2 µg/mL. Samples were incubated with NUP98 polyclonal antibody (Product # PA5-144272). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 198, 188, 98, 97, 196, 187 kDa. Observed band size: 105 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of A549 cells with NUP98 polyclonal antibody (Product # PA5-144272) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of NUP98 in Jurkat whole cell lysate; Lane 1: Rabbit control IgG. Samples were incubated with NUP98 polyclonal antibody (Product # PA5-144272).
