PA5-35364

antibody from Invitrogen Antibodies

Targeting: SCNN1A ENaCalpha, SCNN1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35364

Provider

Invitrogen Antibodies

Product name

alpha-ENaC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SCNN1A in Hela cells using a SCNN1A polyclonal antibody (Product # PA5-35364) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of alpha-ENaC in HeLa cells. Samples were incubated in alpha-ENaC polyclonal antibody (Product # PA5-35364) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of alpha-ENaC in formalin-fixed and paraffin-embedded human lung carcinoma. Samples were incubated with alpha-ENaC polyclonal antibody (Product # PA5-35364) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of SCNN1A in WiDr cells (right) compared to a negative control (left) using a SCNN1A polyclonal antibody (Product # PA5-35364) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of alpha-ENaC in WiDr cells (right histogram). Samples were incubated with alpha-ENaC polyclonal antibody (Product # PA5-35364) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of (overlay histogram) of alpha-ENaC in WiDr cells (green line). Samples were incubated with alpha-ENaC polyclonal antibody (Product # PA5-35364) using a dilution of 1:25 dilution for 60 min at 37°C followed by Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 dilution for 40 min at 37°C. The cells were fixed with 2% paraformaldehyde (10 min). The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the primary antibody. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.