LS-C774305

antibody from LSBio

Targeting: SCNN1A ENaCalpha, SCNN1

Western blot (Enhanced validation)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C774305

Provider

LSBio

Product name

SCNN1A / ENaC Alpha Antibody (clone 2G4, APC) LS-C774305

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Mouse, Rat

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Antibody clone number

2G4

Storage

Store at -20°C.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 µg. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.