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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-47206 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Endocan Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse Endocan is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Endocan in HUVEC human umbilical vein endothelial cells. Samples were incubated in Endocan polyclonal antibody (Product # PA5-47206) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Endocan/ESM‚1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of Endocan in Jurkat human acute T cell leukemia cell line. Samples were incubated in Endocan polyclonal antibody (Product # PA5-47206). Recombinant Human Endocan/ESM‚1, immobilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Endocan/ESM‚1 (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Endocan/ESM‚1 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 1-4 µg/mL.
