Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [3]
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Validation data
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- Product number
- GTX22914 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX22914, RRID:AB_384907
- Product name
- pan Arrestin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat, Bovine
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of pan Arrestin showing staining in the cytoplasm and nucleus of paraffin-treated human cerebellum tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a pan Arrestin polyclonal antibody (GTX22914) diluted by 3% BSA-PBS at a dilution of 1:500 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjμgated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of pan Arrestin showing staining in the cytoplasm and nucleus of paraffin-treated rat brain tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a pan Arrestin polyclonal antibody (GTX22914) diluted by 3% BSA-PBS at a dilution of 1:500 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjμgated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of pan Arrestin showing staining in the cytoplasm and nucleus of paraffin-treated rat spleen tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a pan Arrestin polyclonal antibody (GTX22914) diluted by 3% BSA-PBS at a dilution of 1:500 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjμgated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.