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LearnPAB10016
antibody from Abnova Corporation
Targeting: HMGN1
FLJ27265, FLJ31471, HMG14, MGC104230, MGC117425
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10016 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10016, RRID:AB_1708276
- Product name
- HMGN1 (phospho S20/S24) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of HMGN1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Human non-histone chromosomal protein HMG-17: identification, characterization, chromosome localization and RFLPs of a functional gene from the large multigene family.
Chromosomal protein HMG-14. Identification, characterization, and chromosome localization of a functional gene from the large human multigene family.
Landsman D, McBride OW, Bustin M
Nucleic acids research 1989 Mar 25;17(6):2301-14
Nucleic acids research 1989 Mar 25;17(6):2301-14
Chromosomal protein HMG-14. Identification, characterization, and chromosome localization of a functional gene from the large human multigene family.
Landsman D, McBride OW, Soares N, Crippa MP, Srikantha T, Bustin M
The Journal of biological chemistry 1989 Feb 25;264(6):3421-7
The Journal of biological chemistry 1989 Feb 25;264(6):3421-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using HMGN1 (phospho S20/24) polyclonal antibody (Cat # PAB10016) shows detection of phosphorylated HMGN1 and HMGN2.Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues.Lanes contain : 1 - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 Δ20E, Δ24E, non-phosphorylated, 4 - HMGN1, Δ20E, Δ24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated.Molecular weight markers (not shown) confirm the size of each recombinant protein.The primary antibody was diluted 1 : 1,000 for this experiment.The blot was processed using a 5 sec exposure time.Personal Communication, Yuri Postnikov, NIH, CCR, Bethesda, MD.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of HMGN1 (phospho S20/24) polyclonal antibody (Cat # PAB10016) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X.Tissue was formalin-fixed and paraffin embedded.The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
