AP09300PU-N

antibody from Acris Antibodies GmbH

Targeting: HMGN1 FLJ27265, FLJ31471, HMG14, MGC104230, MGC117425

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09300PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09300PU-N, RRID:AB_2035640

Product name

anti HMGN1 / HMGN2 pSer20/24

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 19-28 of human HMGN protein

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine, Xenopus

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.20 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Affinity Purified anti-HMNG antibody shows detection of phosphorylated HMGN1 and HMGN2. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lanes contain: 1 - HMGN1, nonphosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non- phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry. affinity purified anti-HMGN pS20/pS24 antibody was used at 20 µg/ml to detectsignal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.