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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- GTX85600 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX85600, RRID:AB_10723496
- Product name
- HMG14 (phospho Ser20/Ser24) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of Affinity Purified anti-HMNG antibody shows detection of phosphorylated HMGN1 and HMGN2. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lanes contain: 1. HMGN1, non-phosphorylated 2. HMGN1, phosphorylated 3. HMGN1 delta20E, delta24E, non-phosphorylated 4. HMGN1, delta20E, delta24E, phosphorylated 5. HMGN2, non-phosphorylated 6. HMGN2, phosphorylated Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1000 for this experiment. The blot was procebetaed using a 5 sec exposure time.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human Skin (formalin-fixed, paraffin-embedded) stained with HMGN1 antibody (GTX85600) at 20 μg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
