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- Product number
- PA5-35113 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC25A6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Defining the molecular mechanisms of the mitochondrial permeability transition through genetic manipulation of F-ATP synthase.
Carrer A, Tommasin L, Šileikytė J, Ciscato F, Filadi R, Urbani A, Forte M, Rasola A, Szabò I, Carraro M, Bernardi P
Nature communications 2021 Aug 10;12(1):4835
Nature communications 2021 Aug 10;12(1):4835
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC25A6 in A549 cell lysate (35 µg/lane) using a SLC25A6 polyclonal antibody (Product # PA5-35113).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SLC25A6 in A549 cells using a SLC25A6 polyclonal antibody (Product # PA5-35113) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC25A6 in formalin-fixed, paraffin-embedded human liver tissue using a SLC25A6 polyclonal antibody (Product # PA5-35113) followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of SLC25A6 in A549 cells (right) compared to a negative control (left) using a SLC25A6 polyclonal antibody (Product # PA5-35113) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Characterization of HeLa-Deltag cells. a Western blot analysis of indicated protein in total cell lysates of HeLa-Deltag cells (40 mug/lane). Images are representative of three independent blots. b Western blot analysis of isolated mitochondria from wild-type (WT) and Deltag cells for the indicated F-ATP synthase subunits. Histogram refers to the quantification of protein levels relative to citrate synthase (CS) and represents mean +- SEM of 3 (for subunit a) or 4 (for all other subunits) independent blots, * p < 0.05, two-sided Student's t -test. Gray bars, WT and cyan bars, HeLa-Deltag cells. c Clear native-PAGE analysis and subsequent immunoblotting against ATP synthase subunits beta, c, and against SDHA of WT and Deltag mitochondria in the presence of indicated amount of digitonin (g digitonin/g protein). Images are representative of two independent blots. d Western blot on isolated mitochondria for ANT2 and ANT3. Histogram refers to the quantification of protein levels relative to citrate synthase (CS) and represents mean +- SEM of three independent blots. Two-sided Student's t -test. e Mitochondrial oxygen consumption rate (OCR) was evaluated in intact cells by Seahorse XF Analyzer before and after the addition of oligomycin (O), FCCP (100 nM), rotenone (R), and antimycin A (AA). Traces are average of four independent experiments for WT (gray trace) and Deltag cells (cyan trace). Basal and oligomycin-sensitive OCR is expressed as mean +- SEM of four independent e
