PA5-35113

antibody from Invitrogen Antibodies

Targeting: SLC25A6 ANT3, ANT3Y, MGC17525

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35113

Provider

Invitrogen Antibodies

Product name

SLC25A6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Defining the molecular mechanisms of the mitochondrial permeability transition through genetic manipulation of F-ATP synthase.

Carrer A, Tommasin L, Šileikytė J, Ciscato F, Filadi R, Urbani A, Forte M, Rasola A, Szabò I, Carraro M, Bernardi P
Nature communications 2021 Aug 10;12(1):4835

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC25A6 in A549 cell lysate (35 µg/lane) using a SLC25A6 polyclonal antibody (Product # PA5-35113).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SLC25A6 in A549 cells using a SLC25A6 polyclonal antibody (Product # PA5-35113) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of SLC25A6 in A549 cells (right) compared to a negative control (left) using a SLC25A6 polyclonal antibody (Product # PA5-35113) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 Characterization of HeLa-Deltag cells. a Western blot analysis of indicated protein in total cell lysates of HeLa-Deltag cells (40 mug/lane). Images are representative of three independent blots. b Western blot analysis of isolated mitochondria from wild-type (WT) and Deltag cells for the indicated F-ATP synthase subunits. Histogram refers to the quantification of protein levels relative to citrate synthase (CS) and represents mean +- SEM of 3 (for subunit a) or 4 (for all other subunits) independent blots, * p < 0.05, two-sided Student's t -test. Gray bars, WT and cyan bars, HeLa-Deltag cells. c Clear native-PAGE analysis and subsequent immunoblotting against ATP synthase subunits beta, c, and against SDHA of WT and Deltag mitochondria in the presence of indicated amount of digitonin (g digitonin/g protein). Images are representative of two independent blots. d Western blot on isolated mitochondria for ANT2 and ANT3. Histogram refers to the quantification of protein levels relative to citrate synthase (CS) and represents mean +- SEM of three independent blots. Two-sided Student's t -test. e Mitochondrial oxygen consumption rate (OCR) was evaluated in intact cells by Seahorse XF Analyzer before and after the addition of oligomycin (O), FCCP (100 nM), rotenone (R), and antimycin A (AA). Traces are average of four independent experiments for WT (gray trace) and Deltag cells (cyan trace). Basal and oligomycin-sensitive OCR is expressed as mean +- SEM of four independent e