PA5-29199

antibody from Invitrogen Antibodies

Targeting: SLC25A6 ANT3, ANT3Y, MGC17525

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29199

Provider

Invitrogen Antibodies

Product name

SLC25A6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T. Predicted reactivity: Zebrafish (93%), Pig (96%), Chicken (93%), Sheep (97%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.7 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The RNA-Binding Protein YBX3 Controls Amino Acid Levels by Regulating SLC mRNA Abundance.

Cooke A, Schwarzl T, Huppertz I, Kramer G, Mantas P, Alleaume AM, Huber W, Krijgsveld J, Hentze MW
Cell reports 2019 Jun 11;27(11):3097-3106.e5

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New interaction partners for Nek4.1 and Nek4.2 isoforms: from the DNA damage response to RNA splicing.

Basei FL, Meirelles GV, Righetto GL, Dos Santos Migueleti DL, Smetana JH, Kobarg J
Proteome science 2015;13:11

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC25A6 using 30 µg of 293T lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a SLC25A6 polyclonal antibody (Product # PA5-29199) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC25A6 using Whole cell extracts (30 µg). Samples were loaded onto a 12% SDS-PAGE gel and probed with a SLC25A6 polyclonal antibody (Product # PA5-29199) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SLC25A6 Polyclonal Antibody (Product # PA5-29199). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A6 Polyclonal Antibody (Product # PA5-29199) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SLC25A6 was performed in MCF7 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SLC25A6 Polyclonal Antibody (Product # PA5-29199) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SLC25A6 Polyclonal Antibody detects SLC25A6 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: SLC25A6 stained by SLC25A6 Polyclonal Antibody (Product # PA5-29199) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of SLC25A6 was performed in 293T whole cell extracts using 5 µg of SLC25A6 Polyclonal Antibody (Product # PA5-29199). Samples were transferred to a membrane and probed with SLC25A6 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Nek4 copurifies with the mitochondrial fraction. (A) Nek4 is present in mitochondrial fractions and shows a similar perinuclear pattern of localization (B) such as its putative interactor ANT3, a mitochondrial protein. HEK293 cells were seeded on Cell Carrier 384 well plates coated with polylysine (Perkin Elmer), incubated for two days at 37degC with 5% CO2, then fixed with methanol and stained with proper antibodies. The nuclei were stained with Hoechst. Images were acquired with the Operetta automated microscope (Perkin Elmer) in non-confocal mode using the 60x high NA objective (NA = 0.9). The datasets were imported into Volocity for colocalization analysis and contrast correction. The images are representative of at least 10 replicate experiments. The mitochondrial localization of Nek4 was verified by cell fractioning of HEK293 Flp-In (stably expressing Nek4 isoforms or empty vector - Flag). Using Qproteome Mitochondria Isolation Kit (QIAGEN). N: nuclear, M: mitochondrial and C: cytosolic fractions.