BS1985

antibody from Bioworld Technology, Inc

Targeting: GRK3 ADRBK2, BARK2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

BS1985

Provider

Bioworld Technology, Inc

Proper citation

Bioworld Technology Cat#BS1985, RRID:AB_1662171

Product name

GRK 3 (F391) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide, corresponding to amino acids 380-420 of Human GRK 3.

Description

Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first are the second essenger-regulated kinases such as c-AMP dependent protein kinase A and protein kinase C. The second are the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase, GRK 1; two forms of ÉÇ-adrenergic receptor kinase, GRK 2 (ÉÇARK, ÉÇARK1) and GRK 3 (ÉÇARK2); IT-11 (GRK 4); GRK 5, GRK 6 and GRK 7.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Bioworld Technology, Inc (provider)

Main image

Experimental details

Western blot (WB) analysis of GRK 3 (F391) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264.7 whole cell lysateLane3:PC12 whole cell lysate